SCP2/SCPx Polyklonaler Antikörper

SCP2/SCPx Polyklonal Antikörper für ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB

Konjugation

Unkonjugiert

Publikationen

4

Kat-Nr. : 19182-1-AP

Synonyme

NLTP, NSL TP, SCP 2, SCP chi, SCP X, SCP2, sterol carrier protein 2, Sterol carrier protein X

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Galerie der Validierungsdaten

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  WB analysis of A2780 using 19182-1-AP

  A2780 cells were subjected to SDS PAGE followed by western blot with 19182-1-AP (SCPx antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 19182-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 19182-1-AP (SCPx antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse ovary using 19182-1-AP

  mouse ovary tissue were subjected to SDS PAGE followed by western blot with 19182-1-AP (SCPx antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

Empfohlene Verdünnung

Anwendung	Verdünnung
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 4 publications below

Produktinformation

19182-1-AP bindet in WB SCP2/SCPx und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SCP2/SCPx fusion protein Ag6002
• Vollständiger Name: sterol carrier protein 2
• Berechnetes Molekulargewicht: 59 kDa
• Beobachtetes Molekulargewicht: 46 kDa, 58 kDa
• GenBank-Zugangsnummer: BC067108
• Gene symbol: SCP2
• Gene ID (NCBI): 6342
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SCP2 (Sterol carrier protein-2), also called nonspecific lipid-transfer protein, is thought to play a major role in intracellular lipid transport and metabolism. Mutations of SCP2 have been associated with diseases involving abnormalities in lipid trafficking, such as Zellweger syndrome. The alternative splicing generates 58 kDa SCPx and 13-15 kDa SCP2 proteins, both of which contain a C-terninal SCP2 domain. In addition to the SCP2 domain, SCPx also contains an amino-terminal thiolase domain. The proteolytic cleavage of SCPx occurred when it was imported into peroxisomes, giving rise to a 44-46 kDa thiolase and a 13-15 kDa SCP2. 19182-1-AP antibody recognizes the intact 58 kDa SCPx protein as well as 44-46 kDa thiolase.

Publikationen

Species	Application	Title
	WB	J Biol Chem; A Cargo-centered Perspective on the PEX5 Receptor-mediated Peroxisomal Protein Import Pathway.; Authors - Francisco Tania T
	WB	FEBS J; Membrane topologies of PEX13 and PEX14 provide new insights on the mechanism of protein import into peroxisomes.; Authors - Aurora Barros-Barbosa
rat	WB	J Biol Chem; The peroxisomal matrix protein translocon is a large cavity-forming protein assembly into which PEX5 protein enters to release its cargo.; Authors - Ana F Dias
	WB	Methods Mol Biol; Determining the Topology of Peroxisomal Proteins Using Protease Protection Assays.; Authors - Tânia Francisco