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Mammaglobin A Polyklonaler Antikörper

Mammaglobin A Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 23501-1-AP

Synonyme

hMAM, Mammaglobin 1, Mammaglobin A, MGB1, SCGB2A2, UGB2

Galerie der Validierungsdaten

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  • Western Blot (WB) analysis of MCF-7 cells using Mammaglobin A Polyclonal antibody (23501-1-AP)

    WB analysis of MCF-7 using 23501-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 23501-1-AP (Mammaglobin A antibody at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using Mammaglobin A Polyclonal antibody (23501-1-AP)

    IHC staining of human breast cancer using 23501-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 23501-1-AP (Mammaglobin A antibody at dilution of 1:50.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using Mammaglobin A Polyclonal antibody (23501-1-AP)

    IHC staining of human breast cancer using 23501-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 23501-1-AP (Mammaglobin A antibody at dilution of 1:50.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using Mammaglobin A Polyclonal antibody (23501-1-AP)

    IHC staining of human breast cancer using 23501-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 23501-1-AP (Mammaglobin A antibody at dilution of 1:50.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using Mammaglobin A Polyclonal antibody (23501-1-AP)

    IHC staining of human breast cancer using 23501-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 23501-1-AP (Mammaglobin A antibody at dilution of 1:50.

Geprüfte Anwendungen

Erfolgreiche Detektion in WBMCF-7-Zellen
Erfolgreiche Detektion in IHChumanes Mammakarzinomgewebe
Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:200-1:1000
Immunhistochemie (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

IHCSee 1 publications below

Produktinformation

23501-1-AP bindet in WB, IHC, ELISA Mammaglobin A und zeigt Reaktivität mit human

Getestete Reaktivität human
In Publikationen genannte Reaktivitäthuman
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen Mammaglobin A fusion protein Ag20122
Vollständiger Name secretoglobin, family 2A, member 2
Berechnetes Molekulargewicht 120 aa, 13 kDa
Beobachtetes Molekulargewicht 6-10 kDa
GenBank-ZugangsnummerBC067220
Gene symbol SCGB2A2
Gene ID (NCBI) 4250
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SCGB2A2, also known as mammaglobin A, is a member of the secretoglobin superfamily. It is a breast cancer-associated antigen almost exclusively over-expressed in primary and metastatic human breast cancers, making it a specific molecular marker and a potential therapeutic target for breast cancer. SCGB2A2 is a 93-amino acid protein with a calculated molecular weight of 10.5 kDa. This polyclonal antibody is raised against the C-terminal 71 amino acids of the protein encoded by a cDNA (MGC:71974; BC067220).

Protokolle

Produktspezifische Protokolle
WB protocol for Mammaglobin A antibody 23501-1-APProtokoll herunterladen
IHC protocol for Mammaglobin A antibody 23501-1-APProtokoll herunterladen
Standard-Protokolle
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
humanIHC

Mol Med Rep

Immunolocalization of MMP9 and MMP2 in osteolytic metastasis originating from MDA-MB-231 human breast cancer cells.

Authors - Bo Liu
View Article