Secretogranin III Polyklonaler Antikörper

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• KD/KO Validated

Secretogranin III Polyklonal Antikörper für IHC, IF-P, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, IHC, IF-P, ELISA

Konjugation

Unkonjugiert

Publikationen

12

Kat-Nr. : 10954-1-AP

Synonyme

SCG3, UNQ2502/PRO5990, SgIII, Secretogranin-3, Secretogranin 3

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Galerie der Validierungsdaten

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  IHC staining of mouse pancreas using 10954-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 10954-1-AP (Secretogranin III antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse pancreas using 10954-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 10954-1-AP (Secretogranin III antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas cancer using 10954-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 10954-1-AP (Secretogranin III antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas cancer using 10954-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 10954-1-AP (Secretogranin III antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse pancreas using 10954-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse pancreas tissue using Secretogranin III antibody (10954-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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  IF Staining of mouse pancreas using 10954-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse pancreas tissue using Secretogranin III antibody (10954-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IHC: Maus-Pankreasgewebe, humanes Pankreaskarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: Maus-Pankreasgewebe

Empfohlene Verdünnung

• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 4 publications below
• IHC: See 5 publications below
• IF: See 6 publications below

Produktinformation

10954-1-AP bindet in WB, IHC, IF-P, ELISA Secretogranin III und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Secretogranin III fusion protein Ag1397
• Vollständiger Name: secretogranin III
• Berechnetes Molekulargewicht: 53 kDa
• GenBank-Zugangsnummer: BC014539
• Gene symbol: Secretogranin 3
• Gene ID (NCBI): 29106
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Secretogranin III (SCG3), a member of the granin protein family, is expressed specifically in neuronal and endocrine cells. SCG3 was synthesized as a 61- or 63-kDa protein and processed to a 48-kDa form which, in turn, was partially cleaved to fragments of 28 and 20 kDa.

Publikationen

Species	Application	Title
mouse	WB	J Exp Med; Secretogranin III as a disease-associated ligand for antiangiogenic therapy of diabetic retinopathy.; Authors - Michelle E LeBlanc
human	WB,IHC	Cancer Lett; The oncoprotein HBXIP up-regulates SCG3 through modulating E2F1 and miR-509-3p in hepatoma cells.; Authors - Yue Wang ; KD Validated
mouse	WB,IHC	Cereb Cortex; Secretogranin III is an astrocyte granin that is overexpressed in reactive glia.; Authors - Paco Sonia S
human, mouse	IHC	Brain Pathol; Secretory sorting receptors carboxypeptidase E and secretogranin III in amyloid β-associated neural degeneration in Alzheimer's disease.; Authors - Virginia Plá
mouse	IHC,IF	J Endocrinol; Secretogranin II binds to secretogranin III and forms secretory granules with orexin, neuropeptide Y, and POMC.; Authors - Hotta Kikuko K
	IF,WB	Adv Sci (Weinh); Tuning the Size of Large Dense-Core Vesicles and Quantal Neurotransmitter Release via Secretogranin II Liquid-Liquid Phase Separation.; Authors - Zhaohan Lin ; KD Validated