SCFD1 Polyklonaler Antikörper

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• KD/KO Validated

SCFD1 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen

6

Kat-Nr. : 12569-1-AP

Synonyme

Sly1p, SLY1 homolog, Sec1 family domain-containing protein 1, KIAA0917, FKSG23

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Galerie der Validierungsdaten

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  WB analysis of A549 using 12569-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 12569-1-AP (SCFD1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human liver using 12569-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 12569-1-AP (SCFD1 antibody) at dilution of 1:1200 incubated at room temperature for 1.5 hours.

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  WB analysis using 12569-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12569-1-AP (SCFD1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human kidney using 12569-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 12569-1-AP (SCFD1 antibody) at dilution of 1:1200 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 12569-1-AP

  IP result of anti-SCFD1 (IP:12569-1-AP, 3ug; Detection:12569-1-AP 1:500) with HeLa cells lysate 2150ug.

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  IHC staining of human endometrial cancer using 12569-1-AP

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 12569-1-AP (SCFD1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human endometrial cancer using 12569-1-AP

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 12569-1-AP (SCFD1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human endometrial cancer using 12569-1-AP

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer using 12569-1-AP (SCFD1 antibody) at dilution of 1:50 (under 10x lens).

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  IF Staining of HeLa using 12569-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 12569-1-AP (SCFD1 antibody) at dilution of 1:50 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, HEK-293T-Zellen, humanes Lebergewebe, humanes Nierengewebe, NIH/3T3-Zellen
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Endometriumkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2400
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 6 publications below
• IP: See 1 publications below

Produktinformation

12569-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA SCFD1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Bakterien
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SCFD1 fusion protein Ag3234
• Vollständiger Name: sec1 family domain containing 1
• Berechnetes Molekulargewicht: 642 aa, 72 kDa
• Beobachtetes Molekulargewicht: 66-72 kDa
• GenBank-Zugangsnummer: BC017734
• Gene symbol: SCFD1
• Gene ID (NCBI): 23256
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SCFD1, also named as C14orf163, KIAA0917, STXBP1L2, Sly1p and SLY1, belongs to the STXBP/unc-18/SEC1 family. It is a component of the syntaxin-5 SNARE complex which regulates the early secretory pathway of eukaryotic cells at the level of endoplasmic reticulum (ER) to Golgi transport.(PMID:21242315) SCFD1 plays a role in SNARE-pin assembly and Golgi-to-ER retrograde transport via its interaction with COG4. It is involved in vesicular transport between the endoplasmic reticulum and the Golgi.

Publikationen

Species	Application	Title
human	WB	EMBO J; Direct interaction between the COG complex and the SM protein, Sly1, is required for Golgi SNARE pairing.; Authors - Laufman Orly O
human	WB,IP	Autophagy; Acetylation of SCFD1 regulates SNARE complex formation and autophagosome-lysosome fusion.; Authors - Hong Huang ; KD Validated
human	WB	J Cell Sci; Autophagic substrate clearance requires activity of the syntaxin-5 SNARE complex.; Authors - Renna Maurizio M ; KD Validated
Bacteria	WB	Microb Pathog; Formononetin alleviates Streptococcus suis infection by targeting suilysin.; Authors - Guizhen Wang
	WB	J Appl Microbiol; Silibinin attenuates Streptococcus suis serotype 2 virulence by targeting suilysin.; Authors - Xue Shen
human	WB	Mol Biol Cell; Recruitment of PI4KIIIβ to the Golgi by ACBD3 is dependent on an upstream pathway of a SNARE complex and golgins; Authors - Danièle Stalder ; KO Validated