SCF Polyklonaler Antikörper

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• KD/KO Validated

SCF Polyklonal Antikörper für FC, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

6

Kat-Nr. : 26582-1-AP

Synonyme

c Kit ligand, KIT ligand, Kitl, KITLG, KL 1, Mast cell growth factor, MGF, SCF, SF, SHEP7, Stem cell factor

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Galerie der Validierungsdaten

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  WB analysis of PC-12 using 26582-1-AP

  PC-12 cells were subjected to SDS PAGE followed by western blot with 26582-1-AP (SCF antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse colon using 26582-1-AP

  mouse colon tissue were subjected to SDS PAGE followed by western blot with 26582-1-AP (SCF antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 26582-1-AP

  mouse colon tissue were subjected to SDS PAGE followed by western blot with 26582-1-AP (SCF antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 26582-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 26582-1-AP (SCF antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human kidney using 26582-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 26582-1-AP (SCF antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 26582-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 26582-1-AP (SCF antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 26582-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 26582-1-AP (SCF antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 26582-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 26582-1-AP (SCF antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human small intestine using 26582-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 26582-1-AP (SCF antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human small intestine using 26582-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 26582-1-AP (SCF antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human kidney using 26582-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 26582-1-AP (SCF antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 26582-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 26582-1-AP (SCF antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  FC experiment of MCF-7 using 26582-1-AP

  1X10^6 MCF-7 cells were intracellularly stained with 0.4 ug Anti-Human SCF (26582-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: PC-12-Zellen, Maus-Kolongewebe, NIH/3T3-Zellen
• Erfolgreiche Detektion in IHC: humanes Nierengewebe, humanes Dünndarmgewebe, humanes Kolongewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in FC: MCF-7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Durchflusszytometrie (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IHC: See 4 publications below

Produktinformation

26582-1-AP bindet in WB, IHC, FC, ELISA SCF und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SCF fusion protein Ag25075
• Vollständiger Name: KIT ligand
• Berechnetes Molekulargewicht: 273 aa, 31 kDa
• Beobachtetes Molekulargewicht: 45 kDa
• GenBank-Zugangsnummer: BC074725
• Gene symbol: KITLG
• Gene ID (NCBI): 4254
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Stem cell factor (SCF) also known as mast cell growth factor, steel factor, and kit ligand, is a hematopoietic growth factor. SCF is a critical protein with key roles in the cell such as hematopoiesis, gametogenesis and melanogenesis. SCF activates multiple signal pathways by binding with c-kit receptor. SCF and its receptor, c-kit ligand (KL), are up-regulated in particular human malignancies including gastrointestinal stromal tumor (GISTs), breast cancer, hematopoietic cell, myeloid leukaemia, and glioma. The SCF/c-KIT system also plays a relevant role in cell fate decision, and its activity is widely known to control cell proliferation, differentiation, survival and apoptosis.

Publikationen

Species	Application	Title
human	IHC	Int J Mol Sci; Increase in Chymase-Positive Mast Cells in Recurrent Pleomorphic Adenoma and Carcinoma Ex Pleomorphic Adenoma of the Parotid Gland.; Authors - Ichita Kinoshita
human	IHC	Int J Mol Sci; Increased Chymase-Positive Mast Cells in High-Grade Mucoepidermoid Carcinoma of the Parotid Gland; Authors - Hiromi Nishimura
mouse	WB,IHC	Am J Physiol Gastrointest Liver Physiol; miR-128 participates in the pathogenesis of chronic constipation by regulating the p38α/M-CSF inflammatory signaling pathway.; Authors - Yuntian Hong
mouse	WB,IHC	Exp Ther Med; Arctiin alleviates functional constipation by enhancing intestinal motility in mice; Authors - Yujin Wang
human	WB	Thorac Cancer; High expression of KITLG is a new hallmark activating the MAPK pathway in type A and AB thymoma.; Authors - Zhaoyu Yang ; KD Validated
mouse,human	WB	Chem Biodivers; Shengxuening Extracted from Silkworm Excrement Mitigates Myelosuppression via SCF-Mediated JAK2/STAT3 Signaling.; Authors - Lu Ding