SCAP Polyklonaler Antikörper

SCAP Polyklonal Antikörper für IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen

4

Rezensionen (1)

Kat-Nr. : 12266-1-AP

Synonyme

SREBP cleavage-activating protein, SREBP cleavage activating protein, SREBFchaperone, SREBF chaperone, PSEC0227

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Galerie der Validierungsdaten

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  IP experiment of HEK-293 using 12266-1-AP

  IP result of anti-SCAP (IP:12266-1-AP, 4ug; Detection:12266-1-AP 1:600) with HEK-293 cells lysate 1610 ug.

• 

  IF Staining of HEK-293 using 12266-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using SCAP antibody (12266-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche IP: HEK-293-Zellen
• Erfolgreiche Detektion in IF/ICC: HEK-293-Zellen

Empfohlene Verdünnung

• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 4 publications below

Produktinformation

12266-1-AP bindet in WB, IF/ICC, IP, ELISA SCAP und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SCAP fusion protein Ag2937
• Vollständiger Name: SREBF chaperone
• Berechnetes Molekulargewicht: 1279 aa, 140 kDa
• Beobachtetes Molekulargewicht: 140 kDa
• GenBank-Zugangsnummer: BC020987
• Gene symbol: SCAP
• Gene ID (NCBI): 22937
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SCAP is a regulatory protein that is required for the proteolytic cleavage of the sterol regulatory element-binding protein (SREBP). SCAP is an integral membrane protein located in the endoplasmic reticulum (ER). One of the cytosolic regions of SCAP contains a hexapeptide amino acid sequence, MELADL, that functions to detect cellular cholesterol. When cholesterol is present, SCAP undergoes a conformational change that prevents it from activating SREBP and cholesterol synthesis does not occur. SCAP has 4 isoforms with MW 140, 98, 96 and 85 kDa (refer to UniProt: Q12770).

Publikationen

Species	Application	Title
mouse	WB	Proc Natl Acad Sci U S A; PMID: 24843127; Authors - Chong Wee Liew
human	WB	EMBO Rep; Lin28 enhances de novo fatty acid synthesis to promote cancer progression via SREBP-1.; Authors - Yang Zhang
human	WB	PLoS Pathog; Epstein-Barr virus subverts mevalonate and fatty acid pathways to promote infected B-cell proliferation and survival.; Authors - Liang Wei Wang
human	WB	bioRxiv; Identification of the novel role of sterol regulatory element binding proteins (SREBPs) in mechanotransduction and intraocular pressure regulation; Authors - Ting Wang

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Iram (Verified Customer) (08-14-2020)

Nice staining we got in our tissue sections

• Applications: Immunohistochemistry
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: Human colon cancer tissue