SAFB2 Polyklonaler Antikörper

SAFB2 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Ratte und mehr (1)

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen

1

Kat-Nr. : 11642-1-AP

Synonyme

SAF-B2, SAF B2, KIAA0138

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Galerie der Validierungsdaten

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  WB analysis of MCF-7 using 11642-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 11642-1-AP (SAFB2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat pancreas using 11642-1-AP

  rat pancreas tissue were subjected to SDS PAGE followed by western blot with 11642-1-AP (SAFB2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 11642-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11642-1-AP (SAFB2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 11642-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 11642-1-AP (SAFB2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of BxPC-3 using 11642-1-AP

  BxPC-3 cells were subjected to SDS PAGE followed by western blot with 11642-1-AP (SAFB2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human breast cancer using 11642-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 11642-1-AP (SAFB2 antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of U2OS using 11642-1-AP

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using SAFB2 antibody (11642-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: MCF-7-Zellen, BxPC-3-Zellen, HeLa-Zellen, Ratten-Pankreasgewebe
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: U2OS-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IF: See 1 publications below

Produktinformation

11642-1-AP bindet in WB, IHC, IF/ICC, ELISA SAFB2 und zeigt Reaktivität mit human, Ratten

• Getestete Reaktivität: human, Ratte
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SAFB2 fusion protein Ag2230
• Vollständiger Name: scaffold attachment factor B2
• Berechnetes Molekulargewicht: 528aa,57 kDa; 953aa,107 kDa
• Beobachtetes Molekulargewicht: 130 kDa
• GenBank-Zugangsnummer: BC025279
• Gene symbol: SAFB2
• Gene ID (NCBI): 9667
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SAFB2, also named as Scaffold attachment factor B2 or KIAA0138, is a 953 amino acid protein, which contains one SAP domain and one RRM (RNA recognition motif) domain. SAFB2 expressed at high levels in the CNS and at low levels in the liver. It also is expressed in a wide number of breast cancer cell lines. SAFB2 binds to scaffold/matrix attachment region (S/MAR) DNA and can function as an estrogen receptor corepressor and can also inhibit cell proliferation.

Publikationen

Species	Application	Title
mouse	WB,IF	Proteomics; Establishment of a Proteomic Profile Associated with Gonocyte and Spermatogonial Stem Cell Maturation and Differentiation in Neonatal Mice.; Authors - Bo Zheng