SAE1 Polyklonaler Antikörper

SAE1 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen

5

Kat-Nr. : 10229-1-AP

Synonyme

SUMO-activating enzyme subunit 1, N-terminally processed, SUMO-activating enzyme subunit 1, SUA1, Sentrin/SUMO activating protein AOS1, HSPC140

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Galerie der Validierungsdaten

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  WB analysis of A431 using 10229-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 10229-1-AP (SAE1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 10229-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10229-1-AP (SAE1 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

• 

  WB analysis of COLO 320 using 10229-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 10229-1-AP (SAE1 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 10229-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10229-1-AP (SAE1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 10229-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 10229-1-AP (SAE1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human colon cancer using 10229-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10229-1-AP (SAE1 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human colon cancer using 10229-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10229-1-AP (SAE1 Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human skin cancer using 10229-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 10229-1-AP (SAE1 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human skin cancer using 10229-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 10229-1-AP (SAE1 Antibody) at dilution of 1:200 (under 40x lens).

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  IF Staining of HeLa using 10229-1-AP

  Immunofluorescent analysis of HeLa cells using 10229-1-AP (SAE1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A431-Zellen, A549-Zellen, COLO 320-Zellen, HEK-293-Zellen, HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe, humanes Hautkrebsgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:200-1:1000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 4 publications below
• IHC: See 2 publications below

Produktinformation

10229-1-AP bindet in WB, IHC, IF/ICC, ELISA SAE1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SAE1 fusion protein Ag0275
• Vollständiger Name: SUMO1 activating enzyme subunit 1
• Berechnetes Molekulargewicht: 38 kDa
• Beobachtetes Molekulargewicht: 38 kDa
• GenBank-Zugangsnummer: BC000344
• Gene symbol: SAE1
• Gene ID (NCBI): 10055
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SAE1(SUMO-activating enzyme subunit 1) is also named as AOS1, SUA1, UBLE1A and belongs to the ubiquitin-activating E1 family. SAE1 has a calculated molecular mass of 38 kD. It had an apparent molecular mass of 40 kD by SDS-PAGE. SAE1 and UBA2 form a heterodimer that functions as a SUMO-activating enzyme for the sumoylation of proteins(PMID:9920803). Western blot analysis of synchronized HeLa cells detectes increased AOS1 expression as cells progressed through S phase, followed by a substantial decrease in G2 phase. Immunofluorescence analysis shows AOS1 and UBA2 distributed throughout nuclei, but they are excluded from nucleoli.(PMID:11481243).

Publikationen

Species	Application	Title
mouse	WB	Curr Biol; Pumilio 1 suppresses multiple activators of p53 to safeguard spermatogenesis.; Authors - Dong Chen
human	IHC	Open Med (Wars); Bioinformatics-based analysis of SUMOylation-related genes in hepatocellular carcinoma reveals a role of upregulated SAE1 in promoting cell proliferation.; Authors - Yang Liu
human	WB	Nat Commun; SENP1 prevents steatohepatitis by suppressing RIPK1-driven apoptosis and inflammation; Authors - Lingjie Yan
human	IHC,WB	Curr Issues Mol Biol; SUMO-Activating Enzyme Subunit 1 Is Associated with Poor Prognosis, Tumor Progression, and Radio-Resistance in Colorectal Cancer; Authors - Yueh-Jung Wu
human	WB	Elife; TRIM28 promotes HIV-1 latency by SUMOylating CDK9 and inhibiting P-TEFb.; Authors - Xiancai Ma