SACM1L Polyklonaler Antikörper

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• KD/KO Validated

SACM1L Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen

18

Kat-Nr. : 13033-1-AP

Synonyme

Suppressor of actin mutations 1-like protein, SAC1, Phosphatidylinositol-4-phosphate phosphatase, Phosphatidylinositol-3-phosphatase SAC1, KIAA0851

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Galerie der Validierungsdaten

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  WB analysis of human kidney using 13033-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 13033-1-AP (SACM1L antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 13033-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13033-1-AP (SACM1L antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse lung using 13033-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 13033-1-AP (SACM1L antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 13033-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 13033-1-AP (SACM1L antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 13033-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 13033-1-AP (SACM1L antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse kidney using 13033-1-AP

  IP result of anti-SACM1L (IP:13033-1-AP, 4ug; Detection:13033-1-AP 1:1000) with mouse kidney tissue lysate 4000ug.

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  IHC staining of human brain using 13033-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 13033-1-AP (SACM1L antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human brain using 13033-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 13033-1-AP (SACM1L antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human heart using 13033-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 13033-1-AP (SACM1L Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 13033-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 13033-1-AP (SACM1L Antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of A549 using 13033-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed A549 cells using 13033-1-AP (SACM1L antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Nierengewebe, A549-Zellen, humanes Hirngewebe, Mauslungengewebe, Mausnierengewebe
• Erfolgreiche IP: Mausnierengewebe
• Erfolgreiche Detektion in IHC: humanes Hirngewebe, humanes Herzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: A549-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 6 publications below
• WB: See 15 publications below
• IF: See 6 publications below
• IP: See 1 publications below

Produktinformation

13033-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA SACM1L und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SACM1L fusion protein Ag3682
• Vollständiger Name: SAC1 suppressor of actin mutations 1-like (yeast)
• Berechnetes Molekulargewicht: 587 aa, 67 kDa
• Beobachtetes Molekulargewicht: 60-67 kDa
• GenBank-Zugangsnummer: BC016559
• Gene symbol: SACM1L
• Gene ID (NCBI): 22908
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SACM1L(Suppressor of actin mutations 1-like protein) is also named KIAA0851. It is a phosphoinositide phosphatase that hydrolyzes PtdIns3P and PtdIns4P and has low activity towards PtdIns(3,5)P2.

Publikationen

Species	Application	Title
human,mouse	WB	Cell Res; Intercellular transfer of activated STING triggered by RAB22A-mediated non-canonical autophagy promotes antitumor immunity; Authors - Ying Gao
	IP	Cell Host Microbe; Global mapping of Salmonella enterica-host protein-protein interactions during infection.; Authors - Philipp Walch
mouse	WB	Neuron; An ER Assembly Line of AMPA-Receptors Controls Excitatory Neurotransmission and Its Plasticity.; Authors - Jochen Schwenk
human	WB	Autophagy; PtdIns4P restriction by hydrolase SAC1 decides specific fusion of autophagosomes with lysosomes.; Authors - Hongjun Zhang ; KD Validated
human	WB	Proc Natl Acad Sci U S A; Addiction to Golgi-resident PI4P synthesis in chromosome 1q21.3-amplified lung adenocarcinoma cells.; Authors - Lei Shi
human	WB,IF	J Cell Biol; Sensing of nutrients by CPT1C controls SAC1 activity to regulate AMPA receptor trafficking.; Authors - Maria Casas