S100A7/Psoriasin Polyklonaler Antikörper

S100A7/Psoriasin Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen

9

Kat-Nr. : 13061-1-AP

Synonyme

S100A7, S100 calcium-binding protein A7, Psoriasin, PSOR1, Protein S100-A7

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Galerie der Validierungsdaten

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  WB analysis of MCF-7 using 13061-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 13061-1-AP (S100A7/Psoriasin antibody at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human oesophagus cancer using 13061-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 13061-1-AP (S100A7/Psoriasin antibody at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human oesophagus cancer using 13061-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 13061-1-AP (S100A7/Psoriasin antibody at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human breast cancer using 13061-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13061-1-AP (S100A7/Psoriasin antibody at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human breast cancer using 13061-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13061-1-AP (S100A7/Psoriasin antibody at dilution of 1:50 (under 40x lens).

• 

  IF Staining of MCF-7 using 13061-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using S100A7/Psoriasin antibody (13061-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: MCF-7-Zellen
• Erfolgreiche Detektion in IHC: humanes Ösophaguskarzinomgewebe, humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: MCF-7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:200-1:1000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 7 publications below
• IHC: See 2 publications below
• IF: See 1 publications below

Produktinformation

13061-1-AP bindet in WB, IHC, IF/ICC, ELISA S100A7/Psoriasin und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: S100A7/Psoriasin fusion protein Ag3738
• Vollständiger Name: S100 calcium binding protein A7
• Berechnetes Molekulargewicht: 101 aa, 11 kDa
• Beobachtetes Molekulargewicht: 11 kDa
• GenBank-Zugangsnummer: BC034687
• Gene symbol: S100A7/Psoriasin
• Gene ID (NCBI): 6278
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

S100A7 (psoriasin), a member of the S100 protein family, is a well-known antimicrobial peptide and a signaling molecule which regulates cellular function and is expressed in many squamous cell carcinomas (SCCs), such as SCC of the skin. It is an EF-hand type calcium binding protein localized in epithelial cells, regulates cell proliferation and differentiation. The protein is overexpressed in hyperproliferative skin diseases, exhibits antimicrobial activities against bacteria and induces immunomodulatory activities.

Publikationen

Species	Application	Title
human	WB,IHC	J Eur Acad Dermatol Venereol; TOPK promotes the development of psoriasis and worenine alleviates psoriasiform dermatitis by inhibiting TOPK activity; Authors - Hui Lu
human	WB	J Eur Acad Dermatol Venereol; Differentially expressed proteins identified by TMT proteomics analysis in children with verrucous epidermal naevi.; Authors - T Yuan
human	IHC	BMC Cancer; A novel metabolic subtype with S100A7 high expression represents poor prognosis and immuno-suppressive tumor microenvironment in bladder cancer; Authors - Yun Cai
human	WB	Cell Biol Int; S100A7 promotes the development of human endometriosis by activating NF-κB signaling pathway in endometrial stromal cells.; Authors - Qingqing Sun
human	WB	Oncol Lett; PSMC2, ORC5 and KRTDAP are specific biomarkers for HPV-negative head and neck squamous cell carcinoma.; Authors - Yushen Su
human	WB	Mol Med Rep; TET2 is involved in DNA hydroxymethylation, cell proliferation and inflammatory response in keratinocytes.; Authors - Xinxin Liu