S100A16 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

S100A16 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen

14

Kat-Nr. : 11456-1-AP

Synonyme

Protein S100-F, Protein S100-A16, Protein S100 F, Protein S100 A16, Aging-associated gene 13 protein

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Galerie der Validierungsdaten

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  WB analysis using 11456-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11456-1-AP (S100A16 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 11456-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 11456-1-AP (S100A16 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 11456-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 11456-1-AP (S100A16 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

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  IHC staining of human lung cancer using 11456-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11456-1-AP (S100A16 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human lung cancer using 11456-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11456-1-AP (S100A16 Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human colon cancer using 11456-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11456-1-AP (S100A16 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 11456-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11456-1-AP (S100A16 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse lung using 11456-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 11456-1-AP (S100A16 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 11456-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11456-1-AP (S100A16 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human bowen disease using 11456-1-AP

  Immunohistochemical analysis of paraffin-embedded human bowen disease slide using 11456-1-AP (S100A16 antibody) at dilution of 1:600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human bowen disease using 11456-1-AP

  Immunohistochemical analysis of paraffin-embedded human bowen disease slide using 11456-1-AP (S100A16 antibody) at dilution of 1:600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human bowen disease using 11456-1-AP

  Immunohistochemical analysis of paraffin-embedded human bowen disease slide using 11456-1-AP (S100A16 antibody) at dilution of 1:600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 11456-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11456-1-AP (S100A16 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human lung cancer using 11456-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11456-1-AP (S100A16 Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human colon cancer using 11456-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11456-1-AP (S100A16 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human colon cancer using 11456-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11456-1-AP (S100A16 Antibody) at dilution of 1:200 (under 40x lens).

• 

  IF Staining of MCF-7 using 11456-1-AP

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using S100A16 antibody (11456-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A431-Zellen, DU 145-Zellen, MCF-7-Zellen
• Erfolgreiche Detektion in IHC: humanes Lungenkarzinomgewebe, humanes Kolonkarzinomgewebe, Mauslungengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: MCF-7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 5 publications below
• WB: See 10 publications below
• IHC: See 8 publications below
• IF: See 5 publications below
• IP: See 2 publications below

Produktinformation

11456-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA S100A16 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: S100A16 fusion protein Ag2023
• Vollständiger Name: S100 calcium binding protein A16
• Berechnetes Molekulargewicht: 103 aa, 12 kDa
• Beobachtetes Molekulargewicht: 9-12 kDa
• GenBank-Zugangsnummer: BC019099
• Gene symbol: S100A16
• Gene ID (NCBI): 140576
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

S100A16 is a member of the S100 protein family. S100A16 is expressed in a variety of human tissues. S100A16 expression is especially high in tissues rich in epithelial cells. Functionally, S100A16 has been linked to several aspects of tumorigenesis, for example, cell proliferation, differentiation, migration, invasion, and epithelial-mesenchymal transition (EMT).Accordingly, S100A16 has been suggested to have both tumour-promoting and suppressive roles in human cancers. S100A16-mediated cellular functions are suggested to be mediated by the regulation of various signaling pathways/proteins including EMT-related proteins E-cadherin and Vimentin, PI3K-AKT, p53, MMP1-1, MMP-2, MMP-9, JNK/p38, etc. (PMID: 37509106).

Publikationen

Species	Application	Title
human	WB,IHC,IF	Int J Biol Sci; S100 Calcium Binding Protein A16 Promotes Cell Proliferation by triggering LATS1 ubiquitin degradation mediated by CUL4A ligase to inhibit Hippo pathway in Glioma development; Authors - Yifang Hu ; KD Validated
mouse	WB,IF	Cell Mol Life Sci; S100A16 promotes acute kidney injury by activating HRD1-induced ubiquitination and degradation of GSK3β and CK1α.; Authors - Yifei Sun ; KO Validated
human	WB,IF	FASEB J; Brain microvascular endothelial cell exosome-mediated S100A16 up-regulation confers small-cell lung cancer cell survival in brain.; Authors - Zhi-Hua Xu
human	WB,IP	Front Cell Dev Biol; Calcium Binding Protein S100A16 Expedites Proliferation, Invasion and Epithelial-Mesenchymal Transition Process in Gastric Cancer; Authors - Xiaoying You ; KD Validated
human	IHC, WB	Biomolecules; ADAMTS19 Suppresses Cell Migration and Invasion by Targeting S100A16 via the NF-κB Pathway in Human Gastric Cancer.; Authors - Yingming Jiang
human	WB,IHC,IP	PLoS One; S100A14 Interacts with S100A16 and Regulates Its Expression in Human Cancer Cells.; Authors - Sapkota Dipak D