S100A14 Polyklonaler Antikörper

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• KD/KO Validated

S100A14 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Ratte und mehr (1)

Anwendung

WB, IHC, IF/ICC, IP, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

25

Kat-Nr. : 10489-1-AP

Synonyme

S114, S100A15, S100 calcium-binding protein A14, Protein S100-A14, Protein S100 A14

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Galerie der Validierungsdaten

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  WB analysis using 10489-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10489-1-AP (S100A14 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 10489-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 10489-1-AP (S100A14 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

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  IP experiment of MCF-7 using 10489-1-AP

  IP result of anti-S100A14 (IP:10489-1-AP, 4ug; Detection:10489-1-AP 1:1000) with MCF-7 cells lysate 760 ug.

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  IHC staining of human breast cancer using 10489-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10489-1-AP (S100A14 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 10489-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10489-1-AP (S100A14 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human skin using 10489-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 10489-1-AP (S100A14 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human skin using 10489-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 10489-1-AP (S100A14 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human ovary tumor using 10489-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 10489-1-AP (S100A14 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary tumor using 10489-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 10489-1-AP (S100A14 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 10489-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10489-1-AP (S100A14 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 10489-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10489-1-AP (S100A14 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human gliomas using 10489-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas using 10489-1-AP (S100A14 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human gliomas using 10489-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas using 10489-1-AP (S100A14 antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HeLa using 10489-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using S100A14 antibody (10489-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: MCF-7-Zellen, Ratten-Magengewebe
• Erfolgreiche IP: MCF-7-Zellen
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe, humanes Gliomgewebe, humanes Hautgewebe, humanes Kolonkarzinomgewebe, humanes Ovarialkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 21 publications below
• IHC: See 17 publications below
• IF: See 3 publications below
• IP: See 2 publications below
• CoIP: See 1 publications below

Produktinformation

10489-1-AP bindet in WB, IHC, IF/ICC, IP, CoIP, ELISA S100A14 und zeigt Reaktivität mit human, Ratten

• Getestete Reaktivität: human, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: S100A14 fusion protein Ag0757
• Vollständiger Name: S100 calcium binding protein A14
• Berechnetes Molekulargewicht: 12 kDa
• Beobachtetes Molekulargewicht: 10-12 kDa
• GenBank-Zugangsnummer: BC005019
• Gene symbol: S100A14
• Gene ID (NCBI): 57402
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The S100 family is a multifunctional group of EF-hand type calciumbinding proteins. S100A14 protein (previously known as BCMP84, S100A15) is a recently identified member of the S100 family. It is differentially expressed in a number of different human malignancies like ovary, breast, uterus, kidney, rectum and colon cancers, and esophageal and oral squamous cell carcinomas (OSCCs).

Publikationen

Species	Application	Title
human	WB,IHC	Oncotarget; The role of S100A14 in epithelial ovarian tumors.; Authors - Hanbyoul Cho
human	WB, IHC	Oncogene; S100A14 suppresses metastasis of nasopharyngeal carcinoma by inhibition of NF-kB signaling through degradation of IRAK1.; Authors - Dong-Fang Meng
human	WB, IHC	Int J Cancer; S100A14 is a novel independent prognostic biomarker in the triple-negative breast cancer subtype.; Authors - Sidse Ehmsen
human	WB,IHC,IF	Cell Death Dis; Calcium-binding protein S100A14 induces differentiation and suppresses metastasis in gastric cancer.; Authors - Min Zhu
human	IHC,WB	Am J Cancer Res; A new 7-gene survival score assay for pancreatic cancer patient prognosis prediction.; Authors - Lisi Luo
human	WB,IHC	Eur J Cancer; S100A14 regulates the invasive potential of oral squamous cell carcinoma derived cell-lines in vitro by modulating expression of matrix metalloproteinases, MMP1 and MMP9.; Authors - Sapkota Dipak D ; KD Validated