RTN2 Polyklonaler Antikörper

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• KD/KO Validated

RTN2 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

4

Kat-Nr. : 11168-1-AP

Synonyme

NSP like protein 1, NSP like protein I, NSP2, NSPL1, NSPLI, reticulon 2, RTN2

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Galerie der Validierungsdaten

• 

  WB analysis of mouse skeletal muscle using 11168-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 11168-1-AP (RTN2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human skeletal muscle using 11168-1-AP

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 11168-1-AP (RTN2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skeletal muscle using 11168-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 11168-1-AP (RTN2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skeletal muscle using 11168-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 11168-1-AP (RTN2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human skeletal muscle using 11168-1-AP

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 11168-1-AP (RTN2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human skeletal muscle using 11168-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle slide using 11168-1-AP (RTN2 Antibody) at dilution of 1:50.

• 

  IHC staining of human skeletal muscle using 11168-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle slide using 11168-1-AP (RTN2 Antibody) at dilution of 1:50.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maus-Skelettmuskelgewebe, humanes Skelettmuskelgewebe
• Erfolgreiche Detektion in IHC: humanes Skelettmuskelgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IHC: See 3 publications below
• IP: See 1 publications below

Produktinformation

11168-1-AP bindet in WB, IP, IHC, ELISA RTN2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RTN2 fusion protein Ag1655
• Vollständiger Name: reticulon 2
• Berechnetes Molekulargewicht: 59 kDa, 51 kDa, 22 kDa
• Beobachtetes Molekulargewicht: 20-23 kDa
• GenBank-Zugangsnummer: BC014244
• Gene symbol: RTN2
• Gene ID (NCBI): 6253
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Reticulon (RTN) proteins are a group of membrane-bound proteins that largely reside in endoplasmic reticulum (ER) (PMID: 18177508). Reticulon proteins share a common sequence feature, the reticulon homology domain (RHD). They are involved in shaping the tubular endoplasmic reticulum network, membrane trafficking, inhibition of axonal growth, and apoptosis (PMID: 24218324). Four mammalian reticulons (RTN1-4) exist. Human RTN2 gene produces three isoforms termed RTN2-A, RTN2-B, and RTN2-C. Isoform RTN2-C has a calculated molecular weight of 22 kDa and is highly expressed in skeletal muscle.

Publikationen

Species	Application	Title
human	IHC	Mol Med Rep; RTN2, a new member of circadian clock genes identified by database mining and bioinformatics prediction, is highly expressed in ovarian cancer; Authors - Xiaojiao Zheng
human	WB,IHC	Cell Death Dis; Reticulon 2 promotes gastric cancer metastasis via activating endoplasmic reticulum Ca2+ efflux-mediated ERK signalling.; Authors - Shushu Song ; KD Validated
human	WB,IHC,IP	Cell Signal; O-GlcNAcylation enhances Reticulon 2 protein stability and its promotive effects on gastric cancer progression; Authors - Gaojia Wang
mouse	WB	Nature; Heteromeric clusters of ubiquitinated ER-shaping proteins drive ER-phagy; Authors - Hector Foronda