RRM2 Polyklonaler Antikörper

RRM2 Polyklonal Antikörper für WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

16

Kat-Nr. : 11661-1-AP

Synonyme

RR2M, RR2, R2, EC:1.17.4.1

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Galerie der Validierungsdaten

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  WB analysis using 11661-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11661-1-AP (RRM2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis using 11661-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11661-1-AP (RRM2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IP experiment of K-562 using 11661-1-AP

  IP result of anti-RRM2 (IP:11661-1-AP, 4ug; Detection:11661-1-AP 1:500) with K-562 cells lysate 1200ug.

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  IHC staining of human tonsillitis using 11661-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 11661-1-AP (RRM2 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human tonsillitis using 11661-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 11661-1-AP (RRM2 Antibody) at dilution of 1:50 (under 10x lens).

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  IF Staining of HepG2 using 11661-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using RRM2 antibody (11661-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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  FC experiment of HepG2 using 11661-1-AP

  1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human RRM2 (11661-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, A431-Zellen, HUVEC-Zellen, K-562-Zellen
• Erfolgreiche IP: K-562-Zellen
• Erfolgreiche Detektion in IHC: humanes Tonsillitisgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen
• Erfolgreiche Detektion in FC (Intra): HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 11 publications below
• IHC: See 5 publications below
• IF: See 1 publications below
• CoIP: See 1 publications below

Produktinformation

11661-1-AP bindet in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA RRM2 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Huhn
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RRM2 fusion protein Ag2203
• Vollständiger Name: ribonucleotide reductase M2 polypeptide
• Berechnetes Molekulargewicht: 389 aa, 45 kDa
• Beobachtetes Molekulargewicht: 45 kDa
• GenBank-Zugangsnummer: BC030154
• Gene symbol: RRM2
• Gene ID (NCBI): 6241
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Ribonucleotide reductase M2 subunit is one of two subunits that constitute ribonucleotide reductase, the enzyme that catalyzes the conversion of ribonucleotide 5'-diphosphates into 2'-deoxyribonucleotides, a rate-limiting step in the production of 2'-deoxyribonucleoside 5'-diphosphates (dNTP) required for DNA synthesis and repair that is required for DNA synthesis and repair [PMID:20825972, 19250552]. RRM2 is only expressed during the late G1/early S phase, and degraded in late S phase, and the activity of RNR, and therefore DNA synthesis and cell proliferation, is controlled during the cell cycle by the synthesis and degradation of RRM2 subunit [PMID:3894352].

Publikationen

Species	Application	Title
human	WB	Oxid Med Cell Longev; lncRNA IGF2-AS Regulates Nucleotide Metabolism by Mediating HMGA1 to Promote Pyroptosis of Endothelial Progenitor Cells in Sepsis Patients.; Authors - Guibin Liang
human	IHC	Front Cell Dev Biol; A Novel Ferroptosis-Based Molecular Signature Associated with Biochemical Recurrence-Free Survival and Tumor Immune Microenvironment of Prostate Cancer.; Authors - Zhi-Bin Ke
	WB	Antiviral Res; Long-term HIV-1 infection induces an antiviral state in primary macrophages.; Authors - Maria Pujantell
human	IHC	Front Oncol; Novel Prognostic Signatures of Hepatocellular Carcinoma Based on Metabolic Pathway Phenotypes.; Authors - Tingbo Ye
human	WB	Biomed Pharmacother; Identification of osalmid metabolic profile and active metabolites with anti-tumor activity in human hepatocellular carcinoma cells.; Authors - Zhe Wu
chicken	WB	Poult Sci; RRM2 promotes the proliferation of chicken myoblasts, inhibits their differentiation and muscle regeneration; Authors - Bingjie Chen