RRBP1 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

RRBP1 Polyklonal Antikörper für FC, IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IP, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

6

Kat-Nr. : 22015-1-AP

Synonyme

180 kDa ribosome receptor homolog, ES/130, ES/130 related protein, ES130, hES, KIAA1398, Ribosome binding protein 1, Ribosome receptor protein, RRBP1, RRp

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Galerie der Validierungsdaten

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  WB analysis using 22015-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22015-1-AP (RRBP1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse pancreas using 22015-1-AP

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 22015-1-AP (RRBP1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IHC staining of human lung cancer using 22015-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 22015-1-AP (RRBP1 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 22015-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 22015-1-AP (RRBP1 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 22015-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 22015-1-AP (RRBP1 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas using 22015-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 22015-1-AP (RRBP1 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human pancreas using 22015-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 22015-1-AP (RRBP1 Antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HepG2 using 22015-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using RRBP1 antibody (22015-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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  IF Staining of HepG2 using 22015-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using RRBP1 antibody (22015-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of NIH/3T3 using 22015-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using RRBP1 antibody (22015-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of NIH/3T3 using 22015-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using RRBP1 antibody (22015-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  FC experiment of BxPC-3 using 22015-1-AP

  1X10^6 BxPC-3 cells were stained with 0.2ug RRBP1 antibody (22015-1-AP, red) and control antibody (blue). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500. Cells were fixed with 4% PFA and permeabilized with 0.1% Triton X-100.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HepG2-Zellen, Maus-Pankreasgewebe
• Erfolgreiche Detektion in IHC: humanes Lungenkarzinomgewebe, humanes Pankreasgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: NIH/3T3-Zellen, HepG2-Zellen
• Erfolgreiche Detektion in FC: BxPC-3-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:12000
• Immunhistochemie (IHC): IHC : 1:2000-1:8000
• Immunfluoreszenz (IF): IF : 1:50-1:500
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 4 publications below
• IHC: See 1 publications below
• IF: See 2 publications below
• IP: See 1 publications below

Produktinformation

22015-1-AP bindet in WB, IP, IHC, IF, FC, ELISA RRBP1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RRBP1 fusion protein Ag16648
• Vollständiger Name: ribosome binding protein 1 homolog 180kDa (dog)
• Berechnetes Molekulargewicht: 1410 aa, 152 kDa
• Beobachtetes Molekulargewicht: 180 kDa
• GenBank-Zugangsnummer: BC128577
• Gene symbol: RRBP1
• Gene ID (NCBI): 6238
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Ribosome-binding protein 1 (RRBP1) is an endoplasmic reticulum membrane protein. It is a 1410 amino acid protein composed of a hydrophobic NH2-terminus that includes a transmembrane domain, a highly conserved tandem repeat (ribosome-binding domain), and an acidic coiled-coil COOH-terminal domain. RRBP1 acts as a ribosome receptor and mediates interaction between the ribosome and the endoplasmic reticulum membrane. It is also involved in early cardiac morphogenesis(PMID: 22709790; 8812507; 7686155). RRBP1 has two different isoforms with molecular weights of 150 kDa and 109 kDa.

Publikationen

Species	Application	Title
human	IF	Sci Adv; Oncoprotein SND1 hijacks nascent MHC-I heavy chain to ER-associated degradation, leading to impaired CD8+ T cell response in tumor.; Authors - Yuan Wang
human	WB,IF,IP	Mol Oncol; USP35 mitigates endoplasmic reticulum stress-induced apoptosis by stabilizing RRBP1 in non-small cell lung cancer.; Authors - Wenqing Wang ; KD Validated
		Acta Biochim Biophys Sin (Shanghai); Thymosin beta 4 silencing suppresses proliferation and invasion of non-small cell lung cancer cells by repressing Notch1 activation.; Authors - Dayu Huang
human	WB,IHC	Cancer Manag Res; RRBP1 is highly expressed in prostate cancer and correlates with prognosis.; Authors - Tieqiu Li
human	WB	Science; Endosomal lipid signaling reshapes the endoplasmic reticulum to control mitochondrial function; Authors - Wonyul Jang ; KD Validated
human	WB	Front Oncol; RRBP1 depletion of bone metastatic cancer cells contributes to enhanced expression of the osteoblastic phenotype; Authors - Rui Chen ; KD Validated