RRAS2 Polyklonaler Antikörper

RRAS2 Polyklonal Antikörper für WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Konjugation

Unkonjugiert

Publikationen

3

Kat-Nr. : 12530-1-AP

Synonyme

TC21, Ras-related protein R-Ras2, Ras-like protein TC21, Ras related protein R Ras2, Ras like protein TC21

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Galerie der Validierungsdaten

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  WB analysis of NIH/3T3 using 12530-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 12530-1-AP (RRAS2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse pancreas using 12530-1-AP

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 12530-1-AP (RRAS2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  IP experiment of NIH/3T3 using 12530-1-AP

  IP result of anti-RRAS2 (IP:12530-1-AP, 4ug; Detection:12530-1-AP 1:800) with NIH/3T3 cells lysate 4000ug.

• 

  IHC staining of mouse heart using 12530-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 12530-1-AP (RRAS2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 12530-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 12530-1-AP (RRAS2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 12530-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12530-1-AP (RRAS2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 12530-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12530-1-AP (RRAS2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 12530-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 12530-1-AP (RRAS2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 12530-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 12530-1-AP (RRAS2 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 12530-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 12530-1-AP (RRAS2 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of A431 using 12530-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using RRAS2 antibody (12530-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of A431 using 12530-1-AP

  1X10^6 A431 cells were intracellularly stained with 0.4 ug Anti-Human RRAS2 (12530-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: NIH/3T3-Zellen, Maus-Pankreasgewebe
• Erfolgreiche IP: NIH/3T3-Zellen
• Erfolgreiche Detektion in IHC: Mausherzgewebe, humanes Herzgewebe, humanes Lungenkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: A431-Zellen
• Erfolgreiche Detektion in FC (Intra): A431-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below

Produktinformation

12530-1-AP bindet in WB, IHC, IF/ICC, FC (Intra), IP, ELISA RRAS2 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Affe
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RRAS2 fusion protein Ag3226
• Vollständiger Name: related RAS viral (r-ras) oncogene homolog 2
• Berechnetes Molekulargewicht: 204 aa, 23 kDa
• Beobachtetes Molekulargewicht: 23 kDa
• GenBank-Zugangsnummer: BC013106
• Gene symbol: RRAS2
• Gene ID (NCBI): 22800
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB	Cell Death Differ; Global identification of phospho-dependent SCF substrates reveals a FBXO22 phosphodegron and an ERK-FBXO22-BAG3 axis in tumorigenesis.; Authors - Ping Liu
monkey	WB	Fertil Steril; Long-term effects of repeated superovulation on ovarian structure and function in rhesus monkeys.; Authors - Guoying Dong
human	WB	J Biol Chem; Competition for cysteine acylation by C16:0 and C18:0 derived lipids is a global phenomenon in the proteome; Authors - Hana Nůsková