RPS3 Polyklonaler Antikörper

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• KD/KO Validated

RPS3 Polyklonal Antikörper für WB, IP, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IHC, IF, IP, ELISA

Konjugation

Unkonjugiert

Publikationen

20

Rezensionen (2)

Kat-Nr. : 11990-1-AP

Synonyme

40S ribosomal protein S3, Cell and organelle markers, ribosomal protein S3, Ribosome Marker, RPS3

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Galerie der Validierungsdaten

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  WB analysis using 11990-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11990-1-AP (RPS3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 11990-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 11990-1-AP (RPS3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of PC-12 using 11990-1-AP

  PC-12 cells were subjected to SDS PAGE followed by western blot with 11990-1-AP (RPS3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse testis using 11990-1-AP

  IP result of anti-RPS3 (IP:11990-1-AP, 3ug; Detection:11990-1-AP 1:600) with mouse testis tissue lysate 4000ug.

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  IHC staining of human ovary tumor using 11990-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 11990-1-AP (RPS3 antibody) at dilution of 1:50 (under 10x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HeLa-Zellen, Maushodengewebe, MCF-7-Zellen, NIH/3T3-Zellen, PC-12-Zellen
• Erfolgreiche IP: Maushodengewebe
• Erfolgreiche Detektion in IHC: humanes Ovarialkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 15 publications below
• IHC: See 2 publications below
• IF: See 4 publications below
• IP: See 2 publications below

Produktinformation

11990-1-AP bindet in WB, IHC, IF, IP, ELISA RPS3 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Hausschwein, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RPS3 fusion protein Ag3918
• Vollständiger Name: ribosomal protein S3
• Berechnetes Molekulargewicht: 26 aa, 7 kDa
• Beobachtetes Molekulargewicht: 26.7 kDa
• GenBank-Zugangsnummer: BC034149
• Gene symbol: RPS3
• Gene ID (NCBI): 6188
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

40S ribosomal protein S3 (RPS3), also named as SW-cl.26, is a 243 amino acid protein,which contains one KH type-2 domain and belongs to the ribosomal protein S3P family. RPS3 localizes in the cytoplasm. RPS3 is identified in a IGF2BP1-dependent mRNP granule complex, which contains untranslated mRNAs. RPS3 plays a role in repairing various DNA damage acting as a repair UV endonuclease. Nuclear accumulation of RPS3 results in an increase in DNA repair activity to some extent, thereby sustaining neuronal survival.

Publikationen

Species	Application	Title
human	WB	Nat Commun; Kaposi's sarcoma-associated herpesvirus induces specialised ribosomes to efficiently translate viral lytic mRNAs; Authors - James C Murphy
mouse	IF	Nat Commun; Cilia locally synthesize proteins to sustain their ultrastructure and functions.; Authors - Kai Hao
human	WB	Curr Biol; RPL10L Is Required for Male Meiotic Division by Compensating for RPL10 during Meiotic Sex Chromosome Inactivation in Mice.; Authors - Long Jiang
pig	IF	J Anim Sci Biotechnol; Glyphosate exposure deteriorates oocyte meiotic maturation via induction of organelle dysfunctions in pigs.; Authors - Chunhua Xing
human	WB,IHC	Front Oncol; RPS3 Promotes the Metastasis and Cisplatin Resistance of Adenoid Cystic Carcinoma.; Authors - Xi Rui
human	IF	Front Immunol; Interactome of the Autoimmune Risk Protein ANKRD55.; Authors - Nerea Ugidos

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Boyan (Verified Customer) (12-16-2021)

This antibody recognized a clean band at 27 kd.

• Applications: Western Blot

FH

Huanzhou (Verified Customer) (11-26-2018)

• Applications: Western Blot,
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Cl-16