RPN1 Polyklonaler Antikörper

RPN1 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

13

Kat-Nr. : 12894-1-AP

Synonyme

hrd2, OST1, RBPH1, Ribophorin 1, ribophorin I, RPN I, RPN1

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Galerie der Validierungsdaten

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  WB analysis using 12894-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12894-1-AP (RPN1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 12894-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 12894-1-AP (RPN1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse lung using 12894-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 12894-1-AP (RPN1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse lung using 12894-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 12894-1-AP (RPN1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat liver using 12894-1-AP

  Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 12894-1-AP (RPN1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat liver using 12894-1-AP

  Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 12894-1-AP (RPN1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HT-29-Zellen, HeLa-Zellen, K-562-Zellen
• Erfolgreiche Detektion in IHC: Mauslungengewebe, Rattenlebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:12000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 12 publications below
• IHC: See 2 publications below

Produktinformation

12894-1-AP bindet in WB, IHC, ELISA RPN1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Hefe, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RPN1 fusion protein Ag3576
• Vollständiger Name: ribophorin I
• Berechnetes Molekulargewicht: 69 kDa
• Beobachtetes Molekulargewicht: 66-69 kDa
• GenBank-Zugangsnummer: BC010839
• Gene symbol: RPN1
• Gene ID (NCBI): 6184
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
yeast	WB	Nature; Reconfiguration of the proteasome during chaperone-mediated assembly.; Authors - Soyeon Park
mouse	WB	Nat Cell Biol; Manganese regulation of COPII condensation controls circulating lipid homeostasis; Authors - Xiao Wang
human	WB	Sci Adv; The translocon-associated protein (TRAP) complex regulates quality control of N-linked glycosylation during ER stress.; Authors - Chatchai Phoomak
human	WB	Nat Chem Biol; Oligosaccharyltransferase inhibition induces senescence in RTK-driven tumor cells.; Authors - Cecilia Lopez-Sambrooks
rat	WB	Int J Mol Sci; Impaired 26S Proteasome Assembly Precedes Neuronal Loss in Mutant UBQLN2 Rats.; Authors - Wenjuan Zhang
human	WB	Proteomics; Changes in cellular glycosylation of leukemia cells upon treatment with acridone derivatives yield insight into drug action.; Authors - Yini Wang