RPL7A Polyklonaler Antikörper

RPL7A Polyklonal Antikörper für IF, IHC, IP, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

6

Kat-Nr. : 15340-1-AP

Synonyme

60S ribosomal protein L7a, PLA X polypeptide, ribosomal protein L7a, RPL7A, SURF 3, SURF3, Surfeit locus protein 3, TRUP

---

Galerie der Validierungsdaten

• 

  WB analysis of MCF-7 using 15340-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 15340-1-AP (RPL7A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 15340-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 15340-1-AP (RPL7A Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat kidney using 15340-1-AP

  rat kidney tissue were subjected to SDS PAGE followed by western blot with 15340-1-AP (RPL7A Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 15340-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 15340-1-AP (RPL7A Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 15340-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 15340-1-AP (RPL7A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse kidney using 15340-1-AP

  IP Result of anti-RPL7A (IP:15340-1-AP, 4ug; Detection:15340-1-AP 1:1000) with mouse kidney tissue lysate 4000ug.

• 

  IHC staining of human breast cancer using 15340-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15340-1-AP (RPL7A antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 15340-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15340-1-AP (RPL7A antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 15340-1-AP

  Immunofluorescent analysis of HepG2 cells, using RPL7A antibody 15340-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: MCF7 cells, Mauslebergewebe, Mausnierengewebe, MCF-7-Zellen, Rattennierengewebe
• Erfolgreiche IP: Mausnierengewebe
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 5 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Produktinformation

15340-1-AP bindet in WB, IP, IHC, IF, ELISA RPL7A und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RPL7A fusion protein Ag7543
• Vollständiger Name: ribosomal protein L7a
• Berechnetes Molekulargewicht: 266 aa, 30 kDa
• Beobachtetes Molekulargewicht: 30-32 kDa
• GenBank-Zugangsnummer: BC005128
• Gene symbol: RPL7A
• Gene ID (NCBI): 6130
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Ribosomal proteins are a major component of ribosomes, which catalyze protein synthesis. RPL7a, which is a component of the 60S large ribosomal subunit, has additional functions involved in cell growth and differentiation that occur via interaction with human thyroid hormone receptor (THR) and retinoic acid receptor (RAR) and in turn inhibit the activities of the two nuclear hormone receptors [PMID:21505254].

Publikationen

Species	Application	Title
mouse	WB, IF	Cell; Regnase-1 and Roquin Regulate a Common Element in Inflammatory mRNAs by Spatiotemporally Distinct Mechanisms.; Authors - Takashi Mino
human	IP	Mol Cell; Estrogen-Related Hormones Induce Apoptosis by Stabilizing Schlafen-12 Protein Turnover.; Authors - Dianrong Li
human	WB	Acta Neuropathol Commun; Inefficient quality control of ribosome stalling during APP synthesis generates CAT-tailed species that precipitate hallmarks of Alzheimer's disease; Authors - Suman Rimal
human	WB	Nucleic Acids Res; The induction of p53 correlates with defects in the production, but not the levels, of the small ribosomal subunit and stalled large ribosomal subunit biogenesis; Authors - Matthew John Eastham
human	WB	Am J Cancer Res; UBE2S targets RPL26 for ubiquitination and degradation to promote non-small cell lung cancer progression via regulating c-Myc; Authors - Dalian Gong
mouse	WB	J Exp Med; Phosphorylation-dependent Regnase-1 release from endoplasmic reticulum is critical in IL-17 response.; Authors - Hiroki Tanaka