RPGRIP1 Polyklonaler Antikörper

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• KD/KO Validated

RPGRIP1 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IF, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

1

Kat-Nr. : 13214-1-AP

Synonyme

CORD9, LCA6, RGI1, RGRIP, RPGR interacting protein 1, RPGRIP, RPGRIP1, RPGRIP1d

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Galerie der Validierungsdaten

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  WB analysis using 13214-1-AP

  Y79 cells were subjected to SDS PAGE followed by western blot with 13214-1-AP (RPGRIP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis using 13214-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13214-1-AP (RPGRIP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse eye using 13214-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 13214-1-AP (RPGRIP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse eye using 13214-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 13214-1-AP (RPGRIP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 13214-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 13214-1-AP (RPGRIP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 13214-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 13214-1-AP (RPGRIP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Y79-Zellen, HEK-293-Zellen, HL-60-Zellen
• Erfolgreiche Detektion in IHC: Maus-Augengewebe, Maushodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IF: See 1 publications below

Produktinformation

13214-1-AP bindet in WB, IF, IHC, ELISA RPGRIP1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RPGRIP1 fusion protein Ag4089
• Vollständiger Name: retinitis pigmentosa GTPase regulator interacting protein 1
• Berechnetes Molekulargewicht: 147 kDa
• Beobachtetes Molekulargewicht: 70 kDa
• GenBank-Zugangsnummer: BC039089
• Gene symbol: RPGRIP1
• Gene ID (NCBI): 57096
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RPGRIP1 is a photoreceptor protein that interacts with retinitis pigmentosa GTPase regulator protein and is a key component of cone and rod photoreceptor cells. The biological functions of RPGRIP1 are complex. Mutations in this gene lead to autosomal recessive congenital blindness. In the eye, it is expressed in amacrine neurons and photoreceptors, and in numerous other tissues, albeit at greatly reduced levels. Moreover, it exists multiple isoforms with species-specific subcellular localization patterns, and the different isoforms perform cell-specific functions. Within photoreceptors, RPGRIP1 localizes predominantly to outer segments (OS) of rods and cones in humans, whereas in bovine eyes, it is found only in rod OS. In contrast, in mice, RPGRIP1 is found primarily in connecting cilia (CC), where it localizes between the ciliary axoneme and the plasma membrane. RPGRIP1 has multiple isoforms with MW 147 kDa, 70-76 kDa, 103 kDa, and 66 kDa.

Publikationen

Species	Application	Title
mouse	WB,IF	EMBO J; Cell type-specific regulation of ciliary transition zone assembly in vertebrates.; Authors - Antonia Wiegering ; KO Validated