RPA1 Polyklonaler Antikörper

RPA1 Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

Kat-Nr. : 12448-1-AP

Synonyme

HSSB, MST075, REPA1, Replication factor A protein 1, Replication protein A 70 kDa DNA-binding subunit

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  WB analysis using 12448-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12448-1-AP (RPA1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of Y79 using 12448-1-AP

  Y79 cells were subjected to SDS PAGE followed by western blot with 12448-1-AP (RPA1 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 12448-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 12448-1-AP (RPA1 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

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  WB analysis using 12448-1-AP

  K-562 cell lysates were subjected to SDS PAGE followed by western blot with 12448-1-AP (RPA1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of Y79 using 12448-1-AP

  Y79 cells were subjected to SDS PAGE followed by western blot with 12448-1-AP (RPA1 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, A431-Zellen, HeLa-Zellen, K-562-Zellen, Y79-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

12448-1-AP bindet in WB, ELISA RPA1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RPA1 fusion protein Ag3067
• Vollständiger Name: replication protein A1, 70kDa
• Berechnetes Molekulargewicht: 616 aa, 68 kDa
• Beobachtetes Molekulargewicht: 70 kDa
• GenBank-Zugangsnummer: BC018126
• Gene symbol: RPA1
• Gene ID (NCBI): 6117
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Replication protein A 1 (RPA1) is a subunit of a stable heterotrimer replication protein A (RPA), which is conserved in almost all eukaryotic species (PMID: 27151292). Usually, RPA1, RPA2 and RPA3 function as a whole to participate in DNA replication, telomere maintenance, DNA recombination, DNA repair, and activation of DNA damage checkpoints. In the cell, RPA has a higher affinity to bind single strand DNA and then to form a fork protection complex with other components such as PTEN and OTUB1, thus maintains genome stability during replication(PMID: 26403191; PMID: 18469000).

Publikationen

Species	Application	Title
human	WB	Front Oncol; PMID: 31249811; Authors - Ma-Sha Huang