Kazrin Polyklonaler Antikörper

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• KD/KO Validated

Kazrin Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

1

Kat-Nr. : 11572-1-AP

Synonyme

KAZ, kazrin, KIAA1026, RP1 21O18.1, RP1-21O18.1

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Galerie der Validierungsdaten

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  WB analysis using 11572-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11572-1-AP (kazrin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human heart using 11572-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 11572-1-AP (kazrin antibody) at dilution of 1:1200 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 11572-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11572-1-AP (Kazrin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 11572-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 11572-1-AP (kazrin antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 11572-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 11572-1-AP (kazrin antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat heart using 11572-1-AP

  rat heart tissue were subjected to SDS PAGE followed by western blot with 11572-1-AP (kazrin antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 11572-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 11572-1-AP (kazrin antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of mouse skin using 11572-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 11572-1-AP (kazrin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse skin using 11572-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 11572-1-AP (kazrin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushautgewebe, humanes Herzgewebe, Mausherzgewebe, Maushirngewebe, Rattenhautgewebe, Rattenherzgewebe
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe, Maushautgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IF: See 1 publications below

Produktinformation

11572-1-AP bindet in WB, IF, IHC, ELISA Kazrin und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Kazrin fusion protein Ag2152
• Vollständiger Name: kazrin
• Berechnetes Molekulargewicht: 415 aa, 46 kDa
• Beobachtetes Molekulargewicht: 46 kDa, 37 kDa
• GenBank-Zugangsnummer: BC035501
• Gene symbol: Kazrin
• Gene ID (NCBI): 23254
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Kazrin is an evolutionarily conserved protein that is upregulated during keratinocyte terminal differentiation. Kazrin localizes to desmosomes and binds the epidermal cornified envelope protein periplakin. Kazrin exists three isoforms:kazrin A (46.7 kDa), kazrin B (46 kDa), and kazrin C (37 kDa).

Publikationen

Species	Application	Title
mouse	WB,IF	J Invest Dermatol; Exons 5-15 of kazrin are dispensable for murine epidermal morphogenesis and homeostasis.; Authors - Chhatriwala Mariya K MK ; KD Validated