ROD1 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

ROD1 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

6

Rezensionen (1)

Kat-Nr. : 14027-1-AP

Synonyme

PTBP3, Regulator of differentiation 1, ROD1

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Galerie der Validierungsdaten

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  WB analysis of HL-60 using 14027-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 14027-1-AP (ROD1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 14027-1-AP

  WB result of ROD1 antibody (14027-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ROD1 transfected HeLa cells.

• 

  WB analysis using 14027-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14027-1-AP (ROD1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 14027-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14027-1-AP (ROD1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 14027-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14027-1-AP (ROD1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 14027-1-AP

  Various lysates subjected to SDS PAGE followed by western blot with 14027-1-AP (ROD1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse thymus using 14027-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 14027-1-AP (ROD1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human lymphoma using 14027-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma using 14027-1-AP (ROD1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human lymphoma using 14027-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma using 14027-1-AP (ROD1 antibody) at dilution of 1:100 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HL-60-Zellen, HeLa-Zellen, Jurkat-Zellen, Maus-Thymusgewebe, Ratten-Thymusgewebe
• Erfolgreiche Detektion in IHC: humanes Lymphomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:12000
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 5 publications below
• IHC: See 1 publications below
• CoIP: See 1 publications below

Produktinformation

14027-1-AP bindet in WB, IHC, CoIP, ELISA ROD1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ROD1 fusion protein Ag5172
• Vollständiger Name: ROD1 regulator of differentiation 1 (S. pombe)
• Berechnetes Molekulargewicht: 60 kDa
• Beobachtetes Molekulargewicht: 55-59 kDa
• GenBank-Zugangsnummer: BC044585
• Gene symbol: ROD1
• Gene ID (NCBI): 9991
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ROD1 is a RNA-binding protein that mediates pre-mRNA alternative splicing regulation, it plays a role in the regulation of cell proliferation, differentiation and migration. ROD1 exist some isoforms and also can be modified with the molecular weight range from 55-59 kDa.

Publikationen

Species	Application	Title
human,mouse	WB,CoIP	Cell Res; The RNA-binding protein ROD1/PTBP3 cotranscriptionally defines AID-loading sites to mediate antibody class switch in mammalian genomes.; Authors - Juan Chen ; KO Validated
		iScience; Polypyrimidine tract-binding protein 3/insulin-like growth factor 2 mRNA-binding proteins 3/high-mobility group A1 axis promotes renal cancer growth and metastasis; Authors - Qianqing Wang
human	WB	Cancer Cell Int; PTBP3 regulates proliferation of lung squamous cell carcinoma cells via CDC25A-mediated cell cycle progression.; Authors - Yingji Chen
human	WB,IHC	Cancer Cell Int; PTBP3 promotes migration of non-small cell lung cancer through regulating E-cadherin in EMT signaling pathway.; Authors - Qiong Wu
human	WB	Oncol Lett; Overexpression of ROD1 inhibits invasion of breast cancer cells by suppressing the translocation of β-catenin into the nucleus.; Authors - Ya Zhou
mouse	WB	Mol Cell; PTBP1-activated co-transcriptional splicing controls epigenetic status of pluripotent stem cells; Authors - Camilla Iannone

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Yaroslav (Verified Customer) (08-03-2022)

multiple bands detected at all tested dulitions (1:3000-1:10000)

• Applications: Western Blot
• Primary Antibody Dilution: 1:5000
• Cell Tissue Type: mouse embryonic stem cells

ROD1 Antibody Western Blot validation (1:5000 dilution) in mouse embryonic stem cells (Cat no:14027-1-AP)