ROCK2 Monoklonaler Antikörper

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• KD/KO Validated

ROCK2 Monoklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

6

CloneNo.

1C7B8

Kat-Nr. : 66633-1-Ig

Synonyme

KIAA0619, p164 ROCK 2, Rho kinase 2, ROCK2

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Galerie der Validierungsdaten

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  WB analysis using 66633-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66633-1-Ig (ROCK2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 66633-1-Ig

  WB result of ROCK2 antibody (66633-1-Ig; 1:100000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ROCK2 transfected HeLa cells.

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  IHC staining of human liver cancer using 66633-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66633-1-Ig (ROCK2 antibody) at dilution of 1:500 (under 10x lens).

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  IHC staining of human liver cancer using 66633-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66633-1-Ig (ROCK2 antibody) at dilution of 1:500 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HEK-293-Zellen, Jurkat-Zellen, MCF-7-Zellen, NIH/3T3-Zellen
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 4 publications below
• IHC: See 2 publications below

Produktinformation

66633-1-Ig bindet in WB, IHC, ELISA ROCK2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: ROCK2 fusion protein Ag16330
• Vollständiger Name: Rho-associated, coiled-coil containing protein kinase 2
• Berechnetes Molekulargewicht: 77 kDa, 161 kDa
• Beobachtetes Molekulargewicht: 161 kDa
• GenBank-Zugangsnummer: BC111801
• Gene symbol: ROCK2
• Gene ID (NCBI): 9475
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ROCK2 is a serine/threonine kinase that regulates cytokinesis, smooth muscle contraction, the formation of actin stress fibers and focal adhesions, and the activation of the c-fos serum response element. It is an isozyme of ROCK1, is a target for the small GTPase Rho. ROCK2 is localized in both cytoplasm and nucleus, and the nucleus-localized ROCK2 can target p300 for phosphorylation to regulate its acetyltransferase activity (PMID:16574662).This antibody is specific to ROCK2.

Publikationen

Species	Application	Title
human	WB	Int J Biol Sci; Poly (ADP-ribose) polymerase 1 (PARP1) inhibition promotes pulmonary metastasis of osteosarcoma by boosting ezrin phosphorylation.; Authors - Fangfei Li
mouse	WB	Am J Transl Res; β-catenin regulates myocardial ischemia/reperfusion injury following heterotopic heart transplantation in mice by modulating PTEN pathways.; Authors - Qian Ban
rat	WB	Exp Cell Res; Autophagy regulates exosome secretion in rat nucleus pulposus cells via the RhoC/ROCK2 pathway.; Authors - Shun-Qi Hu ; KD Validated
mouse	IHC	Bioengineered; Terpinen-4-ol inhibits the proliferation and mobility of pancreatic cancer cells by downregulating Rho-associated coiled-coil containing protein kinase 2.; Authors - Wenpeng Cao
human	IHC	Eur J Med Chem; Discovery of unglycosylated indolocarbazoles as ROCK2 isoform-selective inhibitors for the treatment of breast cancer metastasis; Authors - Jinhui Wang
	WB	Exp Eye Res; Fibronectin regulates the self-renewal of rabbit limbal epithelial stem cells by stimulating the Wnt11/Fzd7/ROCK non-canonical Wnt pathway.; Authors - Mingyue Zheng ; KD Validated