RNPS1 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

RNPS1 Polyklonal Antikörper für WB, IP, IF, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IF, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

4

Kat-Nr. : 10555-1-AP

Synonyme

E5.1, LDC2, RNPS1, SR related protein LDC2

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Galerie der Validierungsdaten

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  WB analysis of HEK-293 using 10555-1-AP

  WB result of RNPS1 antibody (10555-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RNPS1 transfected HEK-293 cells.

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  WB analysis of HEK-293 using 10555-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10555-1-AP (RNPS1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 10555-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 10555-1-AP (RNPS1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 10555-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 10555-1-AP (RNPS1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 10555-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10555-1-AP (RNPS1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of Raji using 10555-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 10555-1-AP (RNPS1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 10555-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 10555-1-AP (RNPS1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 10555-1-AP

  IP result of anti-RNPS1 (IP:10555-1-AP, 4ug; Detection:10555-1-AP 1:800) with HEK-293 cells lysate 3200ug.

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  IF Staining of HEK-293 using 10555-1-AP

  Immunofluorescent analysis of HEK-293 cells using 10555-1-AP (RNPS1 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, humanes Hirngewebe, Maushirngewebe, Mausnierengewebe, Raji-Zellen
• Erfolgreiche IP: HEK-293-Zellen
• Erfolgreiche Detektion in IF/ICC: HEK-293-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 3 publications below
• IHC: See 1 publications below
• IF: See 3 publications below
• IP: See 2 publications below

Produktinformation

10555-1-AP bindet in WB, IP, IF, IHC, ELISA RNPS1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RNPS1 fusion protein Ag0844
• Vollständiger Name: RNA binding protein S1, serine-rich domain
• Berechnetes Molekulargewicht: 34 kDa
• Beobachtetes Molekulargewicht: 55-60 kDa
• GenBank-Zugangsnummer: BC001838
• Gene symbol: RNPS1
• Gene ID (NCBI): 10921
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The splicing process of mRNA must be highly precise to carry the correct genetic information to the cytoplasm as templates for translation. And this process involves more than 50 proteins in the splicing complex. RNPS1 is a versatile factor that regulates alternative splicing of a number of pre-mRNAs. It participates in both constitutive splicing and in distinctive modulation of alternative splicing in a substrate dependent manner, and involved in UPF2-dependent nonsense-mediated decay (NMD) of mRNAs containing premature stop codons. Its ser-53 phosphorylation site is important for splicing and translation stimulation activity in vitro

Publikationen

Species	Application	Title
human	WB,IP	Mol Cell; AKT constitutes a signal-promoted alternative exon-junction complex that regulates nonsense-mediated mRNA decay.; Authors - Hana Cho
	IF	J Cell Sci; Novel stress granules-like structures are induced via a paracrine mechanism during viral infection.; Authors - Valentina Iadevaia
mouse	WB,IF	Biochem Biophys Res Commun; RNPS1 inhibition aggravates ischemic brain injury and promotes neuronal death.; Authors - Zhi Zhang ; KD Validated
human	WB,IHC,IF,IP	Int J Oral Sci; RNPS1 stabilizes NAT10 protein to facilitate translation in cancer via tRNA ac4C modification; Authors - Xiaochen Wang ; KD Validated