RNH1 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

RNH1 Polyklonal Antikörper für WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Konjugation

Unkonjugiert

Publikationen

11

Kat-Nr. : 10345-1-AP

Synonyme

RNH, Ribonuclease/angiogenin inhibitor 1, Ribonuclease inhibitor, RAI, PRI

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Galerie der Validierungsdaten

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  WB analysis using 10345-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10345-1-AP (RNH1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa cells using 10345-1-AP

  WB result of RNH1 antibody (10345-1-AP, 1:2500) with si-control and si-RNH1 transfected HeLa cells.

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  WB analysis using 10345-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10345-1-AP (RNH1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 10345-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 10345-1-AP (RNH1 Antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human liver using 10345-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 10345-1-AP (RNH1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 10345-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 10345-1-AP (RNH1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human heart using 10345-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 10345-1-AP (RNH1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of L02 using 10345-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 10345-1-AP (RNH1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 10345-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10345-1-AP (RNH1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse liver using 10345-1-AP

  IP result of anti-RNH1 (IP:10345-1-AP, 4ug; Detection:10345-1-AP 1:500) with mouse liver tissue lysate 6400ug.

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  IP experiment of rat liver using 10345-1-AP

  IP result of anti-RNH1 (IP:10345-1-AP, 4ug; Detection:10345-1-AP 1:8000) with rat liver tissue lysate 5520 ug.

• 

  IP experiment of HEK-293T using 10345-1-AP

  IP result of anti-RNH1 (IP:10345-1-AP, 4ug; Detection:10345-1-AP 1:8000) with HEK-293T cells lysate 2040 ug.

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  IP experiment of mouse liver using 10345-1-AP

  IP result of anti-RNH1 (IP:10345-1-AP, 4ug; Detection:10345-1-AP 1:8000) with mouse liver tissue lysate 1840 ug.

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  IHC staining of mouse liver using 10345-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 10345-1-AP (RNH1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse liver using 10345-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 10345-1-AP (RNH1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of MCF-7 using 10345-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using RNH1 antibody (10345-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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  FC experiment of MCF-7 using 10345-1-AP

  1X10^6 MCF-7 cells were intracellularly stained with 0.4 ug Anti-Human RNH1 (10345-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293T-Zellen, HeLa-Zellen, HepG2-Zellen, humanes Herzgewebe, humanes Hirngewebe, humanes Lebergewebe, humanes Plazenta-Gewebe, Jurkat-Zellen, L02-Zellen
• Erfolgreiche IP: Mauslebergewebe, HEK-293T-Zellen, Rattenlebergewebe
• Erfolgreiche Detektion in IHC: Mauslebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: MCF-7-Zellen
• Erfolgreiche Detektion in FC (Intra): MCF-7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 10 publications below
• IHC: See 2 publications below
• IF: See 2 publications below

Produktinformation

10345-1-AP bindet in WB, IHC, IF/ICC, FC (Intra), IP, ELISA RNH1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RNH1 fusion protein Ag0350
• Vollständiger Name: ribonuclease/angiogenin inhibitor 1
• Berechnetes Molekulargewicht: 50 kDa
• Beobachtetes Molekulargewicht: 45-50 kDa
• GenBank-Zugangsnummer: BC000677
• Gene symbol: RNH1
• Gene ID (NCBI): 6050
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Ribonuclease/ angiogenin inhibitor (RNH, synonyms: RAI, MGC4569, MGC18200, MGC54054) is a protein that binds tightly to ribonucleases in cells and may be essential in the control of mRNA degradation and gene expression. The human RNH gene has been regionally localized to chromosome band 11p15 by in situ hybridization.

Publikationen

Species	Application	Title
mouse	WB	EMBO J; Myeloid cells protect intestinal epithelial barrier integrity through the angiogenin/plexin-B2 axis.; Authors - Rongpan Bai
human	WB	J Cell Biol; Angiogenin cleaves tRNA and promotes stress-induced translational repression.; Authors - Yamasaki Satoshi S ; KD Validated
human	WB	Proc Natl Acad Sci U S A; Sex hormone-dependent tRNA halves enhance cell proliferation in breast and prostate cancers.; Authors - Shozo Honda
human	WB	Int J Mol Sci; RTCB Complex Regulates Stress-Induced tRNA Cleavage; Authors - Yasutoshi Akiyama
human	IHC	J Proteome Res; Post-translational modifications and protein-specific isoforms in endometriosis revealed by 2D DIGE.; Authors - Stephens Andrew N AN
mouse	WB	J Biol Chem; Genome-wide identification and quantitative analysis of cleaved tRNA fragments induced by cellular stress.; Authors - Saikia Mridusmita M