RNF7 Polyklonaler Antikörper

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• KD/KO Validated

RNF7 Polyklonal Antikörper für WB, IF/ICC, IF-P, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IF-P, IP, ELISA

Konjugation

Unkonjugiert

Publikationen

4

Kat-Nr. : 11905-1-AP

Synonyme

SAG, ROC2, RING-box protein 2, RBX2, CKII beta-binding protein 1

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Galerie der Validierungsdaten

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  WB analysis of human liver using 11905-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 11905-1-AP (RNF7 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human testis using 11905-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 11905-1-AP (RNF7 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human heart using 11905-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 11905-1-AP (RNF7 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human skeletal muscle using 11905-1-AP

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 11905-1-AP (RNF7 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human heart using 11905-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 11905-1-AP (RNF7 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse heart using 11905-1-AP

  IP result of anti-RNF7 (IP:11905-1-AP, 4ug; Detection:11905-1-AP 1:600) with mouse heart tissue lysate 4400ug.

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  IF Staining of mouse heart using 11905-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using RNF7 antibody (11905-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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  IF Staining of U2OS using 11905-1-AP

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using RNF7 antibody (11905-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Lebergewebe, humanes Herzgewebe, humanes Hodengewebe, humanes Skelettmuskelgewebe
• Erfolgreiche IP: Mausherzgewebe
• Erfolgreiche Detektion in IF-P: Mausherzgewebe
• Erfolgreiche Detektion in IF/ICC: U2OS-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunfluoreszenz (IF)-P: IF-P : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 3 publications below
• IHC: See 1 publications below

Produktinformation

11905-1-AP bindet in WB, IHC, IF/ICC, IF-P, IP, ELISA RNF7 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RNF7 fusion protein Ag2553
• Vollständiger Name: ring finger protein 7
• Berechnetes Molekulargewicht: 113 aa, 13 kDa
• Beobachtetes Molekulargewicht: 13 kDa
• GenBank-Zugangsnummer: BC008627
• Gene symbol: RNF7
• Gene ID (NCBI): 9616
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RNF (ring finger protein 7 ) is a novel zinc RING finger protein which is redox responsive and protects mammalian cells from apoptosis.It is shown to be localised in the cytoplasmand nucleus of the cell and is expressed in the heart, liver, skeletal muscle predominantly. RNF7 forms oligomers(55 kDa),dimer(28 kDa) and monomer(13 kDa) in the presence of different concentrations of the reductant.

Publikationen

Species	Application	Title
human	WB	Mol Cell Proteomics; Neddylation-Cullin 2-RBX1 E3 ligase axis targets tumor suppressor RhoB for degradation in liver cancer.; Authors - Junfeng Xu ; KD Validated
human	WB	Dev Cell; The UBE2F-CRL5ASB11-DIRAS2 axis is an oncogene and tumor suppressor cascade in pancreatic cancer cells; Authors - Yu Chang
human,mouse	IHC	Nat Aging; Cell type mapping of inflammatory muscle diseases highlights selective myofiber vulnerability in inclusion body myositis; Authors - Sven Wischnewski
human	WB	FASEB J; PMID: 30844312; Authors - Tengyu Ko ; KD Validated