RNF216 Polyklonaler Antikörper

RNF216 Polyklonal Antikörper für WB, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen

1

Kat-Nr. : 10185-1-AP

Synonyme

TRIAD3, Triad domain-containing protein 3, RING-type E3 ubiquitin transferase RNF216, RING finger protein 216, EC:2.3.2.27

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Galerie der Validierungsdaten

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  WB analysis of U2OS using 10185-1-AP

  U2OS cells were subjected to SDS PAGE followed by western blot with 10185-1-AP (RNF216 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of SW480 using 10185-1-AP

  SW480 cells were subjected to SDS PAGE followed by western blot with 10185-1-AP (RNF216 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis using 10185-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10185-1-AP (RNF216 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 10185-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10185-1-AP (RNF216 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IP experiment of SW480 using 10185-1-AP

  IP result of anti-RNF216 (IP:10185-1-AP, 4ug; Detection:10185-1-AP 1:1000) with SW480 cells lysate 1280 ug.

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  IF Staining of A431 using 10185-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using RNF216 antibody (10185-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mausherzgewebe, Mausnierengewebe, Rattenherzgewebe, SW480-Zellen, U2OS-Zellen
• Erfolgreiche IP: SW480-Zellen
• Erfolgreiche Detektion in IF/ICC: A431-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

10185-1-AP bindet in WB, IF/ICC, IP, ELISA RNF216 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RNF216 fusion protein Ag0238
• Vollständiger Name: ring finger protein 216
• Berechnetes Molekulargewicht: 48 kDa
• Beobachtetes Molekulargewicht: 57 kDa, 90-99 kDa
• GenBank-Zugangsnummer: BC000787
• Gene symbol: RNF216
• Gene ID (NCBI): 54476
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RNF216/TRIAD3 is an RBR (RING-between-RING) E3 ligase that encodes for multiple isoforms that include TRIAD3A, TRIAD3B, TRIAD3C and TRIAD3D/E. RNF216 is a broadly expressed RBR E3 ligase, and loss-of-function mutations in RNF216 are linked to the neurode-generative conditions Gordon-Holmes syndrome (GHS) and Huntington-like disorders (PMID: 34998453, 35620441). RNF216 has three different molecular weights, 99 kDa, 106 kDa and 57 kDa, respectively.

Publikationen

Species	Application	Title
human	WB	J Cell Mol Med; Xanthatin synergizes with cisplatin to suppress homologous recombination through JAK2/STAT4/BARD1 axis in human NSCLC cells.; Authors - Jian Zhang