RNF139 Polyklonaler Antikörper

RNF139 Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 28261-1-AP

Synonyme

HRCA1, RCA1, ring finger protein 139, RNF139, TRC8

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Galerie der Validierungsdaten

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  WB analysis using 28261-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 28261-1-AP (RNF139 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HepG2-Zellen, HT-29-Zellen, THP-1-Zellen, U2OS-Zellen, U-87 MG-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

28261-1-AP bindet in WB, ELISA RNF139 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RNF139 fusion protein Ag27147
• Vollständiger Name: ring finger protein 139
• Berechnetes Molekulargewicht: 664 aa, 76 kDa
• Beobachtetes Molekulargewicht: 70 kDa
• GenBank-Zugangsnummer: BC064636
• Gene symbol: RNF139
• Gene ID (NCBI): 11236
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The RNF139 gene (ring-finger protein 139), also known as TRC8 (translocation in renal carcinoma from chromosome 8), was first identified from a family with 3;8 chromosomal translocation and hereditary renal cell carcinoma and thyroid cancer (PMID:22689053). RNF139 encodes an endoplasmic reticulum (ER) protein with 10 transmembrane segments that contains a sterol-sensing domain and  a RING finger motif encoding an E3 ubiquitin ligase (PMID: 19706601). Northern blot and dot blot shows that RNF139 highly expressed in testis, placenta, and adrenal gland and is expressed at lower level in heart, brain,liver,skeletal muscle and pancreas (PMID: 9689122). It is reported that TRC8 suppresses tumorigenesis through targeting heme oxygenase-1 (HO-1), an antioxidant enzyme highly expressed in various cancers, for ubiquitination and degradation (PMID:22689053). TRC8 also involved in cholesterol and fatty acid biosynthesis that are transcriptionally regulated by the sterol response element binding proteins (SREBPs) (PMID:17016439). Acetylation, phosphorylation and autoubiquitination are common post-translational modifications of FNF139 protein.