RNase T2 Polyklonaler Antikörper

RNase T2 Polyklonal Antikörper für IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen

4

Kat-Nr. : 13753-1-AP

Synonyme

RNASET2, RNASE6PL, Ribonuclease T2, Ribonuclease 6, EC:4.6.1.19

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Galerie der Validierungsdaten

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  IP experiment of BxPC-3 using 13753-1-AP

  IP result of anti-RNase T2 (IP:13753-1-AP, 4ug; Detection:13753-1-AP 1:300) with BxPC-3 cells lysate 4000ug.

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  IHC staining of human pancreas cancer using 13753-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 13753-1-AP (RNase T2 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human pancreas cancer using 13753-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 13753-1-AP (RNase T2 antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of transfected cells using 13753-1-AP

  IF result of 13753-1-AP (anti-RNASET2) in COS7 cell by Dr. Nancy Kedersha. COS7 cells transiently transfected with FAST-KD4-YFP, counterstained with PTG RNASET2 antibody (red) and human anti-mitochondrial antigen in blue. Note that FAST-KD4-YFP overexpression alters mitchonrial morphology, and is also present at stress granules (green only).

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Geprüfte Anwendungen

• Erfolgreiche IP: BxPC-3-Zellen
• Erfolgreiche Detektion in IHC: humanes Pankreaskarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: Transfizierte Zellen

Empfohlene Verdünnung

• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IHC: See 1 publications below

Produktinformation

13753-1-AP bindet in IHC, IF/ICC, IP, ELISA RNase T2 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RNase T2 fusion protein Ag4695
• Vollständiger Name: ribonuclease T2
• Berechnetes Molekulargewicht: 256 aa, 29 kDa
• Beobachtetes Molekulargewicht: 29 kDa
• GenBank-Zugangsnummer: BC039713
• Gene symbol: RNase T2
• Gene ID (NCBI): 8635
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RNASET2(Ribonuclease T2) is also named as RNASE6PL(Ribonuclease 6) and belongs to the RNase T2 family. It is a unique member of the growing family of tumor-antagonizing genes and behaves as a class II tumor suppressor and abolish the tumorigenic potential of an ovarian cancer-derived cell line. This protein has 2 isoforms produced by alternative splicing. Both mildly glycosylated (ranging from38 to 45 kDa) and highly glycosylated forms (ranging from 50 to 80 kDa) of human tumor suppressor protein RNASET2 can be detected in immunoblotting in P. Pastoris(PMID: 21446958).

Publikationen

Species	Application	Title
human		FEBS J; Identification of signal peptide features for substrate specificity in human Sec62/Sec63-dependent ER protein import.; Authors - Stefan Schorr
human	IHC	Cell Transplant; Development and Clinical Validation of a Seven-Gene Prognostic Signature Based on Multiple Machine Learning Algorithms in Kidney Cancer.; Authors - Mi Tian
mouse		Cell Signal; Musashi-2 binds with Fbxo6 to induce Rnaset2 ubiquitination and chemokine signaling pathway during vascular smooth muscle cell phenotypic switch in atherosclerosis; Authors - Tao Zhang
human		Nat Commun; PMID: 30842415; Authors - Juanjuan Ou