RNASEH2B Polyklonaler Antikörper

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• KD/KO Validated

RNASEH2B Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

Publikationen(2)

Kat-Nr. : 17527-1-AP

Synonyme

AGS2, DLEU8, Ribonuclease H2 subunit B, ribonuclease H2, subunit B, Ribonuclease HI subunit B, RNase H2 subunit B, RNASEH2B

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  WB analysis of mouse thymus using 17527-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 17527-1-AP (RNASEH2B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse lung using 17527-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 17527-1-AP (RNASEH2B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 17527-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 17527-1-AP (RNASEH2B antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 17527-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 17527-1-AP (RNASEH2B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maus-Thymusgewebe, HeLa-Zellen, MCF-7-Zellen, Mauslungengewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 2 publications below

Produktinformation

17527-1-AP bindet in WB, ELISA RNASEH2B und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RNASEH2B fusion protein Ag11189
• Vollständiger Name: ribonuclease H2, subunit B
• Berechnetes Molekulargewicht: 331 aa, 37 kDa
• Beobachtetes Molekulargewicht: 35 kDa
• GenBank-Zugangsnummer: BC036744
• Gene symbol: RNASEH2B
• Gene ID (NCBI): 79621
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Ribonuclease H2 is the major nuclear enzyme degrading cellular RNA/DNA hybrids in eukaryotes and the sole nuclease known to be able to hydrolyze ribonucleotides misincorporated during genomic replication. RNASEH2B and RNASEH2C are the non-catalytic subunits of RNase H2 complex. Defects in RNASEH2B are a cause of Aicardi-Goutieres syndrome type 2 (AGS2), a genetically heterogeneous disease with clinical features as thrombocytopenia, hepatosplenomegaly and elevated hepatic transaminases along with intermittent fever may erroneously suggest an infective process.

Publikationen

Species	Application	Title
rat	WB	Nutr Metab (Lond); Integrative proteomic and lipidomic analysis of Kaili Sour Soup-mediated attenuation of high-fat diet-induced nonalcoholic fatty liver disease in a rat model.; Authors - Shuo Cong
human	WB	Cell Rep; Pharmacological inhibition of neddylation impairs long interspersed element 1 retrotransposition; Authors - Yan Li ; KD Validated