RMP Polyklonaler Antikörper

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• KD/KO Validated

RMP Polyklonal Antikörper für WB, IP, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IP, IF, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

7

Kat-Nr. : 11277-1-AP

Synonyme

C19orf2, NNX3, PPP1R19, Protein NNX3, RMP, RPB5 mediating protein, URI

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Galerie der Validierungsdaten

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  WB analysis of A549 using 11277-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 11277-1-AP (RMP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 11277-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 11277-1-AP (RMP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 11277-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 11277-1-AP (RMP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 11277-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11277-1-AP (RMP antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 11277-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 11277-1-AP (RMP antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 11277-1-AP

  IP result of anti-RMP (IP:11277-1-AP, 4ug; Detection:11277-1-AP 1:500) with HeLa cells lysate 1600ug.

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  IHC staining of human lymphoma using 11277-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma using 11277-1-AP (RMP antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human liver cancer using 11277-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 11277-1-AP (RMP antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human liver cancer using 11277-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 11277-1-AP (RMP antibody) at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, HEK-293-Zellen, HeLa-Zellen, HepG2-Zellen, Jurkat-Zellen
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Lymphomgewebe, humanes Leberkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 4 publications below
• WB: See 6 publications below
• IHC: See 5 publications below
• IF: See 2 publications below
• IP: See 1 publications below

Produktinformation

11277-1-AP bindet in WB, IP, IF, IHC, ELISA RMP und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RMP fusion protein Ag1789
• Vollständiger Name: chromosome 19 open reading frame 2
• Berechnetes Molekulargewicht: 65 kDa
• Beobachtetes Molekulargewicht: 70-80 kDa
• GenBank-Zugangsnummer: BC026184
• Gene symbol: RMP
• Gene ID (NCBI): 8725
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human,mouse	WB,IHC,IF,IP	Nat Commun; URI alleviates tyrosine kinase inhibitors-induced ferroptosis by reprogramming lipid metabolism in p53 wild-type liver cancers; Authors - Zhiwen Ding ; KD Validated
human	WB	Oncogene; RMP promotes venous metastases of hepatocellular carcinoma through promoting IL-6 transcription.; Authors - J Zhang ; KD Validated
human	WB, IHC	Cell Death Dis; URI regulates tumorigenicity and chemotherapeutic resistance of multiple myeloma by modulating IL-6 transcription.; Authors - J-L Fan ; KD Validated
human	IHC	Oncotarget; RMP predicts survival and adjuvant TACE response in hepatocellular carcinoma.; Authors - Jian Zhang
human	WB	Am J Cancer Res; Silence of URI in gastric cancer cells promotes cisplatin-induced DNA damage and apoptosis; Authors - Huiqin Bian ; KD Validated
human	WB,IHC	Cardiovasc Res; Cardiomyocyte-specific loss of RMP causes myocardial dysfunction and heart failure.; Authors - Jian Zhang