RHPN2 Polyklonaler Antikörper

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• KD/KO Validated

RHPN2 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen

3

Kat-Nr. : 12671-1-AP

Synonyme

Rhophilin-2, Rhophilin 2, RhoBP, p76RBE, GTP-Rho-binding protein 2

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Galerie der Validierungsdaten

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  WB analysis of COLO 320 using 12671-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 12671-1-AP (RHPN2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of COLO 320 using 12671-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 12671-1-AP (RHPN2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 12671-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 12671-1-AP (RHPN2 Antibody) at dilution of 1:600 incubated at 4 degree celsius over night.

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  WB analysis of HepG2 using 12671-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 12671-1-AP (RHPN2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon using 12671-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 12671-1-AP (RHPN2 antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human colon using 12671-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 12671-1-AP (RHPN2 antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human stomach using 12671-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 12671-1-AP (RHPN2 antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human stomach using 12671-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 12671-1-AP (RHPN2 antibody) at dilution of 1:200 (under 40x lens).

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  IF Staining of HepG2 using 12671-1-AP

  Immunofluorescent analysis of HepG2 cells using 12671-1-AP (RHPN2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: COLO 320-Zellen, HepG2-Zellen
• Erfolgreiche Detektion in IHC: humanes Kolongewebe, humanes Magengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IF: See 1 publications below

Produktinformation

12671-1-AP bindet in WB, IHC, IF/ICC, ELISA RHPN2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RHPN2 fusion protein Ag3353
• Vollständiger Name: rhophilin, Rho GTPase binding protein 2
• Berechnetes Molekulargewicht: 686 aa, 77 kDa
• Beobachtetes Molekulargewicht: 77 kDa
• GenBank-Zugangsnummer: BC036447
• Gene symbol: RHPN2
• Gene ID (NCBI): 85415
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RHPN2 binds specifically to GTP-Rho. It may function in a Rho pathway to limit stress fiber formation and/or increase the turnover of F-actin structures in the absence of high levels of RhoA activity. The MW of this protein is 77 kDa, and this antibody specially recognises the 77 kDa protein.

Publikationen

Species	Application	Title
		Sci Signal; RhoA inhibits neural differentiation in murine stem cells through multiple mechanisms.; Authors - Junning Yang
human	WB,IF	Cell Cycle; P76RBE silencing inhibits ovarian cancer cell proliferation, migration, and invasion via suppressing the integrin β1/NF-κB pathway.; Authors - Limei Yan ; KD Validated
mouse	WB	J Biochem Mol Toxicol; Circ_0000284 upregulates RHPN2 to facilitate pancreatic cancer proliferation, metastasis, and angiogenesis through sponging miR-1179; Authors - Jian Zhang