MIRO2 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

MIRO2 Polyklonal Antikörper für WB, IP, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (2)

Anwendung

WB, IP, IF, IHC, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

22

Rezensionen (2)

Kat-Nr. : 11237-1-AP

Synonyme

ARHT2, C16orf39, hMiro 2, MIRO 2, miro2, Mitochondrial Rho GTPase 2, RASL, RHOT2

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Galerie der Validierungsdaten

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  WB analysis using 11237-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11237-1-AP (MIRO2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 11237-1-AP

  WB result of MIRO2 antibody (11237-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MIRO2 transfected HepG2 cells.

• 

  WB analysis using 11237-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11237-1-AP (MIRO2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human heart using 11237-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 11237-1-AP (RHOT2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human kidney using 11237-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 11237-1-AP (RHOT2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 11237-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 11237-1-AP (RHOT2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human testis using 11237-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 11237-1-AP (RHOT2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of L02 using 11237-1-AP

  IP result of anti-MIRO2 (IP:11237-1-AP, 4ug; Detection:11237-1-AP 1:4000) with L02 cells lysate 3080 ug.

• 

  IHC staining of human colon using 11237-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 11237-1-AP (MIRO2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 11237-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 11237-1-AP (MIRO2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 11237-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 11237-1-AP (MIRO2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 11237-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 11237-1-AP (RHOT2 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human kidney using 11237-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 11237-1-AP (RHOT2 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human heart using 11237-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 11237-1-AP (RHOT2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 11237-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 11237-1-AP (RHOT2 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human lung cancer using 11237-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 11237-1-AP (RHOT2 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human lung cancer using 11237-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 11237-1-AP (RHOT2 antibody) at dilution of 1:100 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293T-Zellen, HeLa-Zellen, HepG2-Zellen, humanes Herzgewebe, humanes Hodengewebe, humanes Nierengewebe, K-562-Zellen, L02-Zellen, Mausherzgewebe
• Erfolgreiche IP: L02-Zellen
• Erfolgreiche Detektion in IHC: humanes Kolongewebe, humanes Herzgewebe, humanes Lungenkarzinomgewebe, humanes Nierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:10000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 18 publications below
• IHC: See 1 publications below
• IF: See 6 publications below
• IP: See 4 publications below
• CoIP: See 2 publications below

Produktinformation

11237-1-AP bindet in WB, IP, IF, IHC, CoIP, ELISA MIRO2 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Affe, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MIRO2 fusion protein Ag1752
• Vollständiger Name: ras homolog gene family, member T2
• Berechnetes Molekulargewicht: 68 kDa
• Beobachtetes Molekulargewicht: 80 kDa
• GenBank-Zugangsnummer: BC014942
• Gene symbol: RHOT2
• Gene ID (NCBI): 89941
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RHOT2 (Ras homolog gene family, member T2), also known as ARHT2, RASL or MIRO-2 (mitochondrial Rho GTPase 2), is a 618 amino acid single-pass type IV membrane protein as a member of the Rho family of GTPases. It is localized to the outer mitochondrial membrane and plays a role in mitochondrial trafficking and fusion-fission dynamics. RHOT2 is ubiquitously expressed with highest levels found in pancreas, heart, kidney and skeletal muscle. MIRO2 is highly phosphorylated at basal conditions, as evidenced by an immunoreactive band that electrophoretically migrates 7 kDa higher (~75 kDa) compared to its predicted molecular weight (68 kDa) (PMID: 28556983).

Publikationen

Species	Application	Title
monkey	WB,IF	Nat Cell Biol; MIROs and DRP1 drive mitochondrial-derived vesicle biogenesis and promote quality control.; Authors - Tim König ; KD Validated
		Neuron; Brain-derived autophagosome profiling reveals the engulfment of nucleoid-enriched mitochondrial fragments by basal autophagy in neurons.; Authors - Juliet Goldsmith
	WB	Sci Adv; Global ubiquitylation analysis of mitochondria in primary neurons identifies endogenous Parkin targets following activation of PINK1.; Authors - Odetta Antico
human	IF	EMBO Rep; Parkin-mediated ubiquitylation redistributes MITOL/March5 from mitochondria to peroxisomes.; Authors - Fumika Koyano
human	WB, IP, IF	EMBO Rep; MICAL2PV suppresses the formation of tunneling nanotubes and modulates mitochondrial trafficking.; Authors - Fei Wang
human	WB, IF	Mol Ther Nucleic Acids; miR-351-5p/Miro2 axis contributes to hippocampal neural progenitor cell death via unbalanced mitochondrial fission.; Authors - Ha-Na Woo ; KD Validated

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Mary (Verified Customer) (09-09-2024)

Worked very well so far!!

• Applications: Immunofluorescence

FH

Tim (Verified Customer) (11-25-2021)

Tested on HeLa total cell lysate. Very good.

• Applications: Western Blot
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: HeLa

MIRO2 Antibody Western Blot validation (1/1000 dilution) in HeLa (Cat no:11237-1-AP)