RHCG Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

RHCG Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF, IHC, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

2

Kat-Nr. : 11949-1-AP

Synonyme

Ammonium transporter Rh type C, C15orf6, CDRC2, PDRC2, Rh family type C glycoprotein, Rh family, C glycoprotein, Rh glycoprotein kidney, Rh type C glycoprotein, RHCG, RHGK, SLC42A3, Tumor related protein DRC2

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Galerie der Validierungsdaten

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  WB analysis of HEK-293 using 11949-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 11949-1-AP (RHCG antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of human kidney using 11949-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 11949-1-AP (RHCG antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human pancreas cancer using 11949-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 11949-1-AP (RHCG antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human kidney using 11949-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 11949-1-AP (RHCG antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human kidney using 11949-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 11949-1-AP (RHCG antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human kidney using 11949-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 11949-1-AP (RHCG antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human kidney using 11949-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 11949-1-AP (RHCG antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human kidney using 11949-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 11949-1-AP (RHCG antibody) at dilution of 1:25 (under 10x lens).

• 

  IHC staining of human kidney using 11949-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 11949-1-AP (RHCG antibody) at dilution of 1:25 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, humanes Nierengewebe
• Erfolgreiche Detektion in IHC: humanes Pankreaskarzinomgewebe, humanes Nierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IHC: See 1 publications below
• IF: See 1 publications below
• CoIP: See 1 publications below

Produktinformation

11949-1-AP bindet in WB, IF, IHC, CoIP, ELISA RHCG und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RHCG fusion protein Ag2493
• Vollständiger Name: Rh family, C glycoprotein
• Berechnetes Molekulargewicht: 479 aa, 53 kDa
• Beobachtetes Molekulargewicht: 52-55 kDa
• GenBank-Zugangsnummer: BC030965
• Gene symbol: RHCG
• Gene ID (NCBI): 51458
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
mouse	WB,IF,CoIP	Kidney Int; The ammonia transporter RhCG modulates urinary acidification by interacting with the vacuolar proton-ATPases in renal intercalated cells.; Authors - Soline Bourgeois ; KO Validated
human	WB,IHC	Aging (Albany NY); Rh type C-glycoprotein functions as a novel tumor suppressor gene by inhibiting tumorigenicity and metastasis in head and neck squamous cell carcinoma.; Authors - Wenguang Xu