RGS5 Polyklonaler Antikörper

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• KD/KO Validated

RGS5 Polyklonal Antikörper für WB, IHC, IF/ICC, FC (Intra), ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, FC (Intra), ELISA

Konjugation

Unkonjugiert

Publikationen

14

Kat-Nr. : 11590-1-AP

Synonyme

Regulator of G-protein signaling 5

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Galerie der Validierungsdaten

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  WB analysis using 11590-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11590-1-AP (RGS5 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse skeletal muscle using 11590-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 11590-1-AP (RGS5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human heart using 11590-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 11590-1-AP (RGS5 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 11590-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 11590-1-AP (RGS5 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human skeletal muscle using 11590-1-AP

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 11590-1-AP (RGS5 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 11590-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 11590-1-AP (RGS5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 11590-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 11590-1-AP (RGS5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human stomach cancer using 11590-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 11590-1-AP (RGS5 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 11590-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 11590-1-AP (RGS5 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 11590-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using RGS5 antibody (11590-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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  FC experiment of HeLa using 11590-1-AP

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human RGS5 (11590-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maus-Skelettmuskelgewebe, humanes Herzgewebe, humanes Skelettmuskelgewebe, Mausherzgewebe, MCF-7-Zellen, Ratten-Skelettmuskelgewebe
• Erfolgreiche Detektion in IHC: humanes Magenkrebsgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen
• Erfolgreiche Detektion in FC (Intra): HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:5000
• Immunhistochemie (IHC): IHC : 1:200-1:800
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 7 publications below
• IHC: See 2 publications below
• IF: See 9 publications below

Produktinformation

11590-1-AP bindet in WB, IHC, IF/ICC, FC (Intra), ELISA RGS5 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RGS5 fusion protein Ag2196
• Vollständiger Name: regulator of G-protein signaling 5
• Berechnetes Molekulargewicht: 181 aa, 21 kDa
• Beobachtetes Molekulargewicht: 42 kDa
• GenBank-Zugangsnummer: BC030059
• Gene symbol: RGS5
• Gene ID (NCBI): 8490
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RGS5 inhibits signal transduction by increasing the GTPase activity of G protein alpha subunits thereby driving them into their inactive GDP-bound form. It binds to G(i)-alpha and G(o)-alpha, but not to G(s)-alpha. The 42kd band recognized by this antibody is a RGS5-RGS5 dimer. (Pubmed:17762159)

Publikationen

Species	Application	Title
mouse	WB,IHC	Cell; Beta-Arrestin1 Prevents Preeclampsia by Downregulation of Mechanosensitive AT1-B2 Receptor Heteromers.; Authors - Ursula Quitterer
human	IF	Adv Sci (Weinh); Single-Cell Transcriptomic Analysis of Primary and Metastatic Tumor Ecosystems in Esophageal Squamous Cell Carcinoma; Authors - Yongxu Jia
human	IF	Adv Sci (Weinh); Cancer-Associated Fibroblast-Induced Remodeling of Tumor Microenvironment in Recurrent Bladder Cancer; Authors - Ting Liang
human	IF	Nat Commun; Single-cell RNA sequencing highlights the role of inflammatory cancer-associated fibroblasts in bladder urothelial carcinoma.; Authors - Zhaohui Chen
mouse	WB	Arterioscler Thromb Vasc Biol; HuR (Human Antigen R) Regulates the Contraction of Vascular Smooth Muscle and Maintains Blood Pressure.; Authors - Shanshan Liu
mouse	WB,IF	PLoS One; A regulator of G protein signaling 5 marked subpopulation of vascular smooth muscle cells is lost during vascular disease.; Authors - Ya-Kun Gao