RGS4 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

RGS4 Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

5

Kat-Nr. : 14530-1-AP

Synonyme

RGP4, RGS4, SCZD9

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Galerie der Validierungsdaten

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  WB analysis of mouse cerebellum using 14530-1-AP

  mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 14530-1-AP (RGS4 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 14530-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 14530-1-AP (RGS4 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of PC-12 using 14530-1-AP

  PC-12 cells were subjected to SDS PAGE followed by western blot with 14530-1-AP (RGS4 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 14530-1-AP

  IP Result of anti-RGS4 (IP:14530-1-AP, 4ug; Detection:14530-1-AP 1:300) with mouse brain tissue lysate 4000ug.

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  IHC staining of human lung using 14530-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 14530-1-AP (RGS4 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung using 14530-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 14530-1-AP (RGS4 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human brain using 14530-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 14530-1-AP (RGS4 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human brain using 14530-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 14530-1-AP (RGS4 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 14530-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using RGS4 antibody (14530-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maus-Cerebellum-Gewebe, Maushirngewebe, PC-12-Zellen
• Erfolgreiche IP: Maushirngewebe
• Erfolgreiche Detektion in IHC: humanes Lungengewebe, humanes Hirngewebe, Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IHC: See 3 publications below
• IF: See 1 publications below

Produktinformation

14530-1-AP bindet in WB, IP, IHC, IF, ELISA RGS4 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RGS4 fusion protein Ag6029
• Vollständiger Name: regulator of G-protein signaling 4
• Berechnetes Molekulargewicht: 23 kDa
• Beobachtetes Molekulargewicht: 30 kDa
• GenBank-Zugangsnummer: BC051869
• Gene symbol: RGS4
• Gene ID (NCBI): 5999
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Regulator of G protein signaling (RGS) proteins are a large family of highly diverse, multifunctional signaling proteins. RGS4 is one of seven members of a classic R4 RGS protein that accelerates the GTPase activity of the Gai/o and Gaq/11 family members. RGS4 is highly expressed in multiple brain regions including the cerebral cortex, amygdala, hippocampus, and striatum. Extensive studies have demonstrated that RGS4 plays an important role in regulating smooth muscle contraction, cardiomyocyte development, neural plasticity and psychiatric disorders.

Publikationen

Species	Application	Title
human,mouse	IHC	J Allergy Clin Immunol; RGS4 promotes allergen- and aspirin-associated airway hyperresponsiveness by inhibiting PGE2 biosynthesis.; Authors - Gordon S Wong ; KO Validated
mouse	WB	Arterioscler Thromb Vasc Biol; HuR (Human Antigen R) Regulates the Contraction of Vascular Smooth Muscle and Maintains Blood Pressure.; Authors - Shanshan Liu
human	WB,IHC	Oncotarget; Regulator of G protein signaling 4 inhibits human melanoma cells proliferation and invasion through the PI3K/AKT signaling pathway.; Authors - Xiaotong Xue
human	WB,IHC,IF	Onco Targets Ther; RGS4 Regulates Proliferation And Apoptosis Of NSCLC Cells Via microRNA-16 And Brain-Derived Neurotrophic Factor.; Authors - Zheng He
human	WB	Arch Gerontol Geriatr; LINC00370 modulates miR-222-3p-RGS4 axis to protect against osteoporosis progression.; Authors - Lintao Li