RGS14 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

RGS14 Polyklonal Antikörper für IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (2)

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

7

Kat-Nr. : 16258-1-AP

Synonyme

RGS14

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Galerie der Validierungsdaten

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  WB analysis using 16258-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16258-1-AP (RGS14 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 16258-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 16258-1-AP (RGS14 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse spleen using 16258-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 16258-1-AP (RGS14 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 16258-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 16258-1-AP (RGS14 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 16258-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 16258-1-AP (RGS14 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 16258-1-AP

  IP Result of anti-RGS14 (IP:16258-1-AP, 3ug; Detection:16258-1-AP 1:1000) with mouse brain tissue lysate 5000ug.

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  IHC staining of mouse brain using 16258-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 16258-1-AP (RGS14 antibody) at dilution of 1:5000 (under 4x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 16258-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 16258-1-AP (RGS14 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HepG2-Zellen, HuH-7-Zellen, Maushirngewebe, Maushodengewebe, Mauslebergewebe, Mausmilzgewebe
• Erfolgreiche IP: Maushirngewebe
• Erfolgreiche Detektion in IHC: Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:2500-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IHC: See 2 publications below
• IF: See 4 publications below
• IP: See 1 publications below

Produktinformation

16258-1-AP bindet in WB, IP, IHC, IF, ELISA RGS14 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Affe, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RGS14 fusion protein Ag9292
• Vollständiger Name: regulator of G-protein signaling 14
• Berechnetes Molekulargewicht: 566 aa, 61 kDa
• Beobachtetes Molekulargewicht: 60-65 kDa
• GenBank-Zugangsnummer: BC014094
• Gene symbol: RGS14
• Gene ID (NCBI): 10636
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RGS14, a member of the R12 subfamily of RGS proteins, is highly expressed in the brain and is a natural suppressor of CA2 hippocampal synaptic plasticity and learning and memory. RGS14 was first identified as a complex scaffolding protein with an unconventional domain structure that allows it to interact with various protein binding partners. RGS14 contains one RGS domain, two Raf-like Ras-binding domains (RBDs), and one GoLoco domain. The protein attenuates the signaling activity of G-proteins by binding, through its GoLoco domain, to specific types of activated, GTP-bound G alpha subunits. Acting as a GTPase activating protein (GAP), the protein increases the rate of conversion of the GTP to GDP.

Publikationen

Species	Application	Title
human,monkey	WB,IHC	Brain Struct Funct; Regulator of G protein signaling 14 (RGS14) is expressed pre- and postsynaptically in neurons of hippocampus, basal ganglia, and amygdala of monkey and human brain.; Authors - Katherine E Squires
rat	IHC	Exp Neurol; Modelling cognitive deficits in Parkinson's disease: Is CA2 a gateway for hippocampal synucleinopathy?; Authors - Elif Cinar
human	WB, IF	J Biol Chem; Human genetic variants disrupt RGS14 nuclear shuttling and regulation of LTP in hippocampal neurons.; Authors - Katherine E Squires
human	WB,IF	J Biol Chem; RGS14 regulates hormone-sensitive NPT2A-mediated renal phosphate uptake via binding to the NHERF1 scaffolding protein.; Authors - Peter A Friedman
mouse	IP	J Biol Chem; 14-3-3γ binds RGS14 at distinct sites to inhibit the RGS14:Gαi-AlF4- signaling complex and RGS14 nuclear localization.; Authors - Kyle J Gerber
human,mouse,rat	WB,IF	PLoS One; Endogenous RGS14 is a cytoplasmic-nuclear shuttling protein that localizes to juxtanuclear membranes and chromatin-rich regions of the nucleus.; Authors - Mary Rose Branch ; KO Validated