RFC2 Polyklonaler Antikörper

RFC2 Polyklonal Antikörper für WB, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen

5

Rezensionen (1)

Kat-Nr. : 10410-1-AP

Synonyme

RF C 40 kDa subunit, Replication factor C subunit 2, Activator 1 subunit 2, Activator 1 40 kDa subunit, A1 40 kDa subunit

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Galerie der Validierungsdaten

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  WB analysis of HeLa using 10410-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10410-1-AP (RFC2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 10410-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10410-1-AP (RFC2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 10410-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 10410-1-AP (RFC2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Raji using 10410-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 10410-1-AP (RFC2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 10410-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10410-1-AP (RFC2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 10410-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10410-1-AP (RFC2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 10410-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10410-1-AP (RFC2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 10410-1-AP

  IP result of anti-RFC2 (IP:10410-1-AP, 4ug; Detection:10410-1-AP 1:500) with HeLa cells lysate 3000 ug.

• 

  IF Staining of HeLa using 10410-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 10410-1-AP (RFC2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, MCF-7-Zellen, Raji-Zellen
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 5 publications below

Produktinformation

10410-1-AP bindet in WB, IF/ICC, IP, ELISA RFC2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RFC2 fusion protein Ag0663
• Vollständiger Name: replication factor C (activator 1) 2, 40kDa
• Berechnetes Molekulargewicht: 40 kDa
• Beobachtetes Molekulargewicht: 35-40 kDa
• GenBank-Zugangsnummer: BC002813
• Gene symbol: RFC2
• Gene ID (NCBI): 5982
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RFC2, also named as RFC40, belongs to the activator 1 small subunits family. It is a 40kd Activator 1 subunit. It binds ATP. The elongation of primed DNA templates by DNA polymerase delta and epsilon requires the action of the accessory proteins proliferating cell nuclear antigen (PCNA) and activator 1. RFC2 is a constitutively expressed protein essential for both DNA repair and replication. (PMID: 20660762).

Publikationen

Species	Application	Title
mouse	WB	Mol Syst Biol; The MHC I immunopeptidome conveys to the cell surface an integrative view of cellular regulation.; Authors - Caron Etienne E
mouse	WB	Proc Natl Acad Sci U S A; Deletion of TDP-43 down-regulates Tbc1d1, a gene linked to obesity, and alters body fat metabolism.; Authors - Chiang Po-Min PM
human	WB	Sci Rep; RFC2: a prognosis biomarker correlated with the immune signature in diffuse lower-grade gliomas.; Authors - Xu Zhao
human	WB	bioRxiv; Arginine shortage induces replication stress and confers genotoxic resistance by inhibiting histone H4 translation and promoting PCNA polyubiquitination; Authors - Yi-Chang Wang
human	WB	Cell Rep; Arginine shortage induces replication stress and confers genotoxic resistance by inhibiting histone H4 translation and promoting PCNA ubiquitination; Authors - Yi-Chang Wang

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Lei (Verified Customer) (11-29-2018)

This antibody works well in WB. For IP, 50% glycerol contain interferes with antibody-protein A bead binding.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: HepG2

RFC2 Antibody Western Blot, validation (1:500 dilution) in HepG2 (Cat no:10410-1-AP)