Renin receptor, ATP6AP2 Polyklonaler Antikörper

Renin receptor, ATP6AP2 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen

6

Kat-Nr. : 10926-1-AP

Synonyme

ATP6AP2, Renin receptor, APT6M8 9, ATPase H(+)-transporting lysosomal accessory protein 2, ATP6M8-9

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Galerie der Validierungsdaten

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  WB analysis of human retinal pigment epithelium tissue using 10926-1-AP

  WB result from Oscar Alcazar, et al, (Pro)renin receptor is expressed in human retinal pigment epithelium and participates in extracellular matrix remodeling, Exp Eye Res. 89(5) 638-47 (2009) (PMID:19580809). Eye 45kd.

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  WB analysis using 10926-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10926-1-AP (Renin receptor, ATP6AP2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human heart using 10926-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 10926-1-AP (Renin receptor, ATP6AP2 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human heart using 10926-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 10926-1-AP (Renin receptor, ATP6AP2 Antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HeLa using 10926-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Renin receptor, ATP6AP2 antibody (10926-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, humanes Pigmentepithel-Gewebe, Rattenhirngewebe
• Erfolgreiche Detektion in IHC: humanes Herzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 6 publications below
• IHC: See 3 publications below

Produktinformation

10926-1-AP bindet in WB, IHC, IF/ICC, ELISA Renin receptor, ATP6AP2 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Renin receptor, ATP6AP2 fusion protein Ag1360
• Vollständiger Name: ATPase, H+ transporting, lysosomal accessory protein 2
• Berechnetes Molekulargewicht: 39 kDa
• Beobachtetes Molekulargewicht: 28 kDa
• GenBank-Zugangsnummer: BC010395
• Gene symbol: Renin receptor
• Gene ID (NCBI): 10159
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ATP6AP2, also named as ATP6IP2, CAPER, ELDF10, N14F, ATP6M8-9, Renin receptor, and prorenin receptor, is believed to potentiate the renin-angiotensin system (RAS), conferring to prorenin, a likely pathological role at the tissue level. The PRR has been identified in the microvascular endothelial cells of the retina, which seems to be involved in pathological neovascularization processes. The present study demonstrates for the first time that the PRR is expressed in human ATP6AP2 and suggests a molecular mechanism by which hypertension may exacerbate the pathology of dry AMD. ATP6AP2 functions as a renin and prorenin cellular receptor. It may mediate renin-dependent cellular responses by activating ERK1 and ERK2. By increasing the catalytic efficiency of renin in AGT/angiotensinogen conversion to angiotensin I, it may also play a role in the renin-angiotensin system (RAS). Defects in ATP6AP2 are a cause of mental retardation X-linked with epilepsy (MRXE). The full length of ATP6AP2 protein is 39 kDa, and the band with an apparent molecular weight of 28 kDa is the soluble form.(PMID:19580809; PMID:28215051; PMID:34534267; PMID: 29127204)

Publikationen

Species	Application	Title
mouse	WB	J Clin Invest; mTORC1 feedback to AKT modulates lysosomal biogenesis through MiT/TFE regulation.; Authors - Kaushal Asrani
human	WB,IHC	Exp Eye Res; (Pro)renin receptor is expressed in human retinal pigment epithelium and participates in extracellular matrix remodeling.; Authors - Alcazar Oscar O
human	WB	Int J Clin Exp Pathol; MicroRNA-140-3p inhibits proliferation, migration and invasion of lung cancer cells by targeting ATP6AP2.; Authors - Xiao-Mei Kong
human	WB,IHC	Cell Biosci; PABPN1 regulates mRNA alternative polyadenylation to inhibit bladder cancer progression; Authors - Liang Chen
mouse	WB,IHC	Placenta; Endothelin-1 production via placental (pro)renin receptor in a mouse model of preeclampsia; Authors - Sakurako Mishima
mouse	WB	ACS Nano; Acidic Nanoparticles Restore Lysosomal Acidification and Rescue Metabolic Dysfunction in Pancreatic β-Cells under Lipotoxic Conditions; Authors - Chih Hung Lo