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RELB Polyklonaler Antikörper

RELB Polyklonal Antikörper für FC (Intra), IF

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

IF, FC (Intra)

Konjugation

CoraLite® Plus 488 Fluorescent Dye

Kat-Nr. : CL488-25027

Synonyme

I REL, IREL, RELB, Transcription factor RelB

Galerie der Validierungsdaten

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CoraLite® Plus 488-conjugated RELB Polyclonal anti (CL488-25027)

    IF Staining of HeLa using CL488-25027

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 RELB antibody (CL488-25027) at dilution of 1:200.

  • Flow cytometry (FC) experiment of Jurkat cells using CoraLite® Plus 488-conjugated RELB Polyclonal anti (CL488-25027)

    FC experiment of Jurkat using CL488-25027

    1X10^6 Jurkat cells were intracellularly stained with 0.2 ug CoraLite® Plus 488 Anti-Human RELB (CL488-25027) (red), or 0.2 ug Mouse IgG1 Isotype Control (CL488-66360, Clone: T1F8D3F10) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

Geprüfte Anwendungen

Erfolgreiche Detektion in IFHeLa-Zellen
Erfolgreiche Detektion in FCJurkat-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Immunfluoreszenz (IF)IF : 1:50-1:500
Durchflusszytometrie (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

CL488-25027 bindet in IF, FC (Intra) RELB und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen RELB fusion protein Ag13824
Vollständiger Name v-rel reticuloendotheliosis viral oncogene homolog B
Berechnetes Molekulargewicht 62 kDa
Beobachtetes Molekulargewicht 62-65 kDa
GenBank-ZugangsnummerBC028013
Gene symbol RELB
Gene ID (NCBI) 5971
Konjugation CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer BS mit 50% Glyzerin, 0,05% Proclin300, 0,5% BSA, pH 7,3.
LagerungsbedingungenBei -20°C lagern. Vor Licht schützen. Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RELB, also named as Transcription factor RelB, is a 579 amino acid protein, which contains one RHD domain. RELB localizes in the nucleus and cytoplasm. RELB is a pleiotropic transcription factor which is present in almost all cell types and is involved in many biological processed such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis.

Protokolle

Produktspezifische Protokolle
IF protocol for CL Plus 488 RELB antibody CL488-25027Protokoll herunterladen
FC protocol for CL Plus 488 RELB antibody CL488-25027Protokoll herunterladen
Standard-Protokolle
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen