REC8 Polyklonaler Antikörper

REC8 Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (2)

Anwendung

WB, IF, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

14

Kat-Nr. : 10793-1-AP

Synonyme

Cohesin Rec8p, HR21spB, REC8, REC8 homolog (yeast), REC8L1, Rec8p

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Galerie der Validierungsdaten

• 

  WB analysis of human testis using 10793-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 10793-1-AP (REC8 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 10793-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 10793-1-AP (REC8 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human testis using 10793-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 10793-1-AP (REC8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Hodengewebe, K-562-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 7 publications below
• IHC: See 1 publications below
• IF: See 7 publications below

Produktinformation

10793-1-AP bindet in WB, IF, IHC, ELISA REC8 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: REC8 fusion protein Ag1250
• Vollständiger Name: REC8 homolog (yeast)
• Berechnetes Molekulargewicht: 63 kDa
• Beobachtetes Molekulargewicht: 85-95 kDa
• GenBank-Zugangsnummer: BC010887
• Gene symbol: REC8
• Gene ID (NCBI): 9985
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

REC8, also named as Rec8p and REC8L1, belongs to the rad21 family. This cohesin protein is required during meiosis for separation of sister chromatids and homologous chromosomes. Proteolytic cleavage of REC8 on chromosome arms by separin during anaphase I allows for homologous chromosome separation in meiosis I and cleavage of REC8 on centromeres during anaphase II allows for sister chromatid separation in meiosis II. Rec8 is present in an unphosphorylated form early in meiotic prophase but is phosphorylated prior to meiosis I. This antibody is a rabbit polyclonal antibody raised against residues near the C terminus of human REC8. This antibody can recognize the monomeric, non-phosphorylated form(63-70kd) of REC8 and other forms(80-100kd) which represent either posttranslationally modified forms or alternative transcript products (PMID: 12615909,19463812).

Publikationen

Species	Application	Title
human	WB	Clin Cancer Res; Ectopic Expression of Cancer-Testis Antigens in Cutaneous T-cell Lymphoma Patients.; Authors - Ivan V Litvinov
mouse	IF	Cell Cycle; Knockout of cyclin B1 in granulosa cells causes female subfertility.; Authors - Jinmei Cheng
		Cell Cycle; Elevated intracellular pH appears in aged oocytes and causes oocyte aneuploidy associated with the loss of cohesion in mice.; Authors - Jin-Mei Cheng
mouse,rat	WB	Biomed Pharmacother; Ephedra herb reduces adriamycin-induced testicular toxicity by upregulating the gonadotropin-releasing hormone signalling pathway.; Authors - Yang Fu
human	IF	Int J Mol Sci; "Mitotic Slippage" and Extranuclear DNA in Cancer Chemoresistance: A Focus on Telomeres.; Authors - Kristine Salmina
human	WB	Exp Cell Res; The role of meiotic cohesin REC8 in chromosome segregation in gamma irradiation-induced endopolyploid tumour cells.; Authors - Erenpreisa Jekaterina J