RCN2 Polyklonaler Antikörper

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• KD/KO Validated

RCN2 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

12

Kat-Nr. : 10193-2-AP

Synonyme

ERC 55, E6BP, E6-binding protein, E6 binding protein, Calcium-binding protein ERC-55

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Galerie der Validierungsdaten

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  WB analysis using 10193-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10193-2-AP (RCN2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 10193-2-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 10193-2-AP (RCN2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis using 10193-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10193-2-AP (RCN2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse lung using 10193-2-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 10193-2-AP (RCN2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse lung using 10193-2-AP

  IP result of anti-RCN2 (IP:10193-2-AP, 4ug; Detection:10193-2-AP 1:400) with mouse lung tissue lysate 2800ug.

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  IHC staining of human gliomas using 10193-2-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas using 10193-2-AP (RCN2 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human gliomas using 10193-2-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas using 10193-2-AP (RCN2 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human gliomas using 10193-2-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas using 10193-2-AP (RCN2 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human gliomas using 10193-2-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas using 10193-2-AP (RCN2 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human gliomas using 10193-2-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas using 10193-2-AP (RCN2 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human testis using 10193-2-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 10193-2-AP (RCN2 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human testis using 10193-2-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 10193-2-AP (RCN2 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human testis using 10193-2-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 10193-2-AP (RCN2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 10193-2-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 10193-2-AP (RCN2 antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HeLa using 10193-2-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using RCN2 antibody (10193-2-AP) at dilution of 1:700 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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  IF Staining of HepG2 using 10193-2-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using RCN2 antibody (10193-2-AP) at dilution of 1:700 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HeLa-Zellen, humanes Hirngewebe, Mauslungengewebe
• Erfolgreiche IP: Mauslungengewebe
• Erfolgreiche Detektion in IHC: humanes Gliomgewebe, humanes Hodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen, HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:350-1:1400

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 9 publications below
• IHC: See 5 publications below
• IF: See 4 publications below
• CoIP: See 1 publications below

Produktinformation

10193-2-AP bindet in WB, IHC, IF/ICC, IP, CoIP, ELISA RCN2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RCN2 fusion protein Ag0247
• Vollständiger Name: reticulocalbin 2, EF-hand calcium binding domain
• Berechnetes Molekulargewicht: 37 kDa
• Beobachtetes Molekulargewicht: 55 kDa
• GenBank-Zugangsnummer: BC004892
• Gene symbol: RCN2
• Gene ID (NCBI): 5955
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Reticulocalbin 2 (RCN2) is a Ca(2+)-binding protein that is specifically localized in the endoplasmic reticulum (ER). It is also termed ERC-55, i.e. ER calcium-binding protein of 55 kDa. It is a single copy gene and is encoded by an approximately 1900-base mRNA, which shows a ubiquitous expression pattern. The protein comprises an amino-terminal signal sequence followed by six copies of the EF-hand Ca2+ binding motif.

Publikationen

Species	Application	Title
mouse	WB	Nat Med; Chemical corrector treatment ameliorates increased seizure susceptibility in a mouse model of familial epilepsy.; Authors - Norihiko Yokoi
rat	WB	Cell Metab; A mechanosensitive lipolytic factor in the bone marrow promotes osteogenesis and lymphopoiesis.; Authors - Hui Peng
mouse	WB,IF	Proc Natl Acad Sci U S A; Stk40 links the pluripotency factor Oct4 to the Erk/MAPK pathway and controls extraembryonic endoderm differentiation.; Authors - Li Lingjie L ; KD Validated
human	IF	Elife; Live-cell mapping of organelle-associated RNAs via proximity biotinylation combined with protein-RNA crosslinking.; Authors - Pornchai Kaewsapsak
human	IF	Elife; Proteomic mapping of cytosol-facing outer mitochondrial and ER membranes in living human cells by proximity biotinylation.; Authors - Victoria Hung
human	WB	Am J Cancer Res; YY1-mediated reticulocalbin-2 upregulation promotes the hepatocellular carcinoma progression via activating MYC signaling.; Authors - Chengjie Mei