RCC1 Polyklonaler Antikörper

RCC1 Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

1

Kat-Nr. : 22335-1-AP

Synonyme

Cell cycle regulatory protein, CHC1, RCC1, RCC1 I

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Galerie der Validierungsdaten

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  WB analysis using 22335-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22335-1-AP (RCC1 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 22335-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 22335-1-AP (RCC1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 22335-1-AP

  IP Result of anti-RCC1 (IP:22335-1-AP, 3ug; Detection:22335-1-AP 1:1500) with HeLa cells lysate 3000ug.

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  IHC staining of mouse testis using 22335-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 22335-1-AP (RCC1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 22335-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 22335-1-AP (RCC1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human small intestine using 22335-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 22335-1-AP (RCC1 antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human small intestine using 22335-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 22335-1-AP (RCC1 antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human testis using 22335-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 22335-1-AP (RCC1 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human testis using 22335-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 22335-1-AP (RCC1 antibody) at dilution of 1:50 (under 10x lens).

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  IF Staining of HepG2 using 22335-1-AP

  Immunofluorescent analysis of HepG2 cells using 22335-1-AP (RCC1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: SH-SY5Y-Zellen, HepG2-Zellen, Maus-Kolongewebe, Ratten-Kolongewebe
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: Maushodengewebe, humanes Dünndarmgewebe, humanes Hodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:5000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IHC: See 1 publications below

Produktinformation

22335-1-AP bindet in WB, IP, IHC, IF, ELISA RCC1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RCC1 fusion protein Ag17809
• Vollständiger Name: regulator of chromosome condensation 1
• Berechnetes Molekulargewicht: 421 aa, 45 kDa
• Beobachtetes Molekulargewicht: 45-50 kDa
• GenBank-Zugangsnummer: BC010067
• Gene symbol: RCC1
• Gene ID (NCBI): 1104
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CHC1, also named as RCC1, promotes the exchange of ran-bound gdp by gtp. It is involved in the regulation of onset of chromosome condensation in the S-phase. Phosphorylation of RCC1 on serines located in or near its nuclear localization signal activates RCC1 to generate RanGTP on mitotic chromosomes, which is required for spindle assembly and chromosome segregation. This antibody is a rabbit polyclonal antibody raised against full length RCC1 of human origin.

Publikationen

Species	Application	Title
human	IHC	Pathol Oncol Res; RCC1 Expression as a Prognostic Marker in Colorectal Liver Oligometastases.; Authors - Yuxiang Deng