p107 Polyklonaler Antikörper

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• KD/KO Validated

p107 Polyklonal Antikörper für WB, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IP, ELISA

Konjugation

Unkonjugiert

Publikationen

14

Kat-Nr. : 13354-1-AP

Synonyme

CP107, p107, PRB1, RBL1, retinoblastoma like 1 (p107), Retinoblastoma like protein 1

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Galerie der Validierungsdaten

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  WB analysis of mouse testis using 13354-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 13354-1-AP (p107 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of COLO 320 using 13354-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 13354-1-AP (p107 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse testis using 13354-1-AP

  IP result of anti-p107 (IP:13354-1-AP, 4ug; Detection:13354-1-AP 1:500) with mouse testis tissue lysate 4000ug.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushodengewebe, COLO 320-Zellen
• Erfolgreiche IP: Maushodengewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 14 publications below
• IP: See 1 publications below

Produktinformation

13354-1-AP bindet in WB, IP, ELISA p107 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, nicht-humane Primaten
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: p107 fusion protein Ag4146
• Vollständiger Name: retinoblastoma-like 1 (p107)
• Berechnetes Molekulargewicht: 1068 aa, 119 kDa
• Beobachtetes Molekulargewicht: 119 kDa
• GenBank-Zugangsnummer: BC032247
• Gene symbol: p107
• Gene ID (NCBI): 5933
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Retinoblastoma-like protein 1, also named RBL1, p107, is a member of E1A-binding proteins, which contains a A/B pocket, or pRb. Via this structure, RBL1 can bind to viral oncoproteins and other perteins containing LXCXE motif. It regulates cell proliferation via phosphorylation-sensitive interactions with E2F transcription factors and other proteins. Key regulator of entry into cell division. Directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation. Recruits and targets histone methyltransferases SUV420H1 and SUV420H2, leading to epigenetic transcriptional repression.(uniprot). It has been reported there are two transcirpts variants of RBL1, which encode a 119-kd protein and a 68-kd protein(PMID:7490090).

Publikationen

Species	Application	Title
mouse	WB,IP	Nat Commun; p107 mediated mitochondrial function controls muscle stem cell proliferative fates.; Authors - Debasmita Bhattacharya
human	WB	Cancers (Basel); RBL1/p107 Expression Levels Are Modulated by Multiple Signaling Pathways.; Authors - Elisa Ventura
human	WB	Front Cell Dev Biol; miR-29b-3p Increases Radiosensitivity in Stemness Cancer Cells via Modulating Oncogenes Axis; Authors - Dong Pan
human	WB	Oncotarget; Methylation of promoter of RBL1 enhances the radioresistance of three dimensional cultured carcinoma cells.; Authors - Dong Pan ; KD Validated
mouse	WB	Biochem Pharmacol; Ribociclib mitigates cisplatin-associated kidney injury through retinoblastoma-1 dependent mechanisms.; Authors - Ji Young Kim
human	WB	Mol Pharm; Synergetic and Antagonistic Molecular Effects Mediated by the Feedback Loop of p53 and JNK between Saikosaponin D and SP600125 on Lung Cancer A549 Cells.; Authors - Xiaoman Chen