RBBP8 Polyklonaler Antikörper

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• KD/KO Validated

RBBP8 Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

Publikationen

4

Kat-Nr. : 12624-1-AP

Synonyme

CtBP interacting protein, CTIP, DNA endonuclease RBBP8, RBBP 8, RBBP8, RIM, SAE2

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Galerie der Validierungsdaten

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  WB analysis of Raji using 12624-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 12624-1-AP (RBBP8 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 12624-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 12624-1-AP (RBBP8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Raji-Zellen, A431-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 4 publications below

Produktinformation

12624-1-AP bindet in WB, ELISA RBBP8 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RBBP8 fusion protein Ag3314
• Vollständiger Name: retinoblastoma binding protein 8
• Berechnetes Molekulargewicht: 103 kDa
• Beobachtetes Molekulargewicht: 120 kDa
• GenBank-Zugangsnummer: BC030590
• Gene symbol: RBBP8
• Gene ID (NCBI): 5932
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RBBP8 is an endonuclease that cooperates with the MRE11-RAD50-NBN (MRN) complex in processing meiotic and mitotic double-strand breaks (DSBs) by ensuring both resection and intrachromosomal association of the broken ends. It functions downstream of the MRN complex and ATM, promotes ATR activation and its recruitment to DSBs in the S/G2 phase facilitating the generation of ssDNA. It interacted with BRCA1 to form a complex that regulates CHEK1 activation and controls cell cycle G2/M checkpoints on DNA damage. Also it promotes microhomology-mediated alternative end joining (A-NHEJ) during class-switch recombination and plays an essential role in chromosomal translocations

Publikationen

Species	Application	Title
human	WB	Oncogene; RBBP8/CtIP suppresses P21 expression by interacting with CtBP and BRCA1 in gastric cancer.; Authors - Yang Yu ; KD Validated
human	WB	J Biol Chem; PTIP UFMylation promotes replication fork degradation in BRCA1-deficient cells; Authors - Qunsong Tan
human	WB	Cell Biochem Funct; The CTIP-mediated repair of TNF-α-induced DNA double-strand break was impaired by miR-130b in cervical cancer cell.; Authors - Lei Yang
human	WB	Cell Biosci; Modulation of cell cycle increases CRISPR-mediated homology-directed DNA repair; Authors - Guoling Li