RBBP5 Polyklonaler Antikörper

RBBP5 Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

Publikationen

1

Kat-Nr. : 13181-1-AP

Synonyme

RBBP 5, RBBP5, RBQ3, SWD1

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Galerie der Validierungsdaten

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  WB analysis of HL-60 using 13181-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 13181-1-AP (RBBP5 Antibody) at dilution of 1:600 incubated at 4 degree celsius over night.

• 

  WB analysis of HL-60 using 13181-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 13181-1-AP (RBBP5 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HL-60 using 13181-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 13181-1-AP (RBBP5 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 13181-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 13181-1-AP (RBBP5 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HL-60 using 13181-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 13181-1-AP (RBBP5 Antibody) at dilution of 1:600 incubated at 4 degree celsius over night.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HL-60-Zellen, HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

13181-1-AP bindet in WB, ELISA RBBP5 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RBBP5 fusion protein Ag3848
• Vollständiger Name: retinoblastoma binding protein 5
• Berechnetes Molekulargewicht: 538 aa, 59 kDa
• Beobachtetes Molekulargewicht: 59 kDa
• GenBank-Zugangsnummer: BC037284
• Gene symbol: RBBP5
• Gene ID (NCBI): 5929
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RBQ-3, also known as RBBP5 (retinoblastoma binding protein 5) or SWD1, is a 538 amino acid protein that localizes to the nucleus and contains six WD repeats. Expressed ubiquitously, RBQ-3 functions as a component of the Set1 complex and preferentially binds to underphosphorylated forms of the retinoblastoma (Rb) protein, possibly playing a role in the regulation of cell proliferation. RBQ-3 exists as two alternatively spliced isoforms and, upon DNA damage, is subject to phosphorylation by ATM or ATR.

Publikationen

Species	Application	Title
human	WB	Nat Commun; Menin enhances c-Myc-mediated transcription to promote cancer progression.; Authors - Gongwei Wu