RB1CC1 Polyklonaler Antikörper

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• KD/KO Validated

RB1CC1 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen

2

Kat-Nr. : 10069-1-AP

Synonyme

RBICC, RB1-inducible coiled-coil protein 1, RB1CC 1, KIAA0203, FAK family kinase-interacting protein of 200 kDa

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Galerie der Validierungsdaten

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  WB analysis of HEK-293 using 10069-1-AP

  WB result of RB1CC1 antibody (10069-1-AP, 1:1000) with si-Control and si-RB1CC1 transfected HEK293 cells.

• 

  WB analysis of MCF-7 using 10069-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 10069-1-AP (RB1CC1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skeletal muscle using 10069-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 10069-1-AP (RB1CC1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 10069-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10069-1-AP (RB1CC1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 10069-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10069-1-AP (RB1CC1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 10069-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10069-1-AP (RB1CC1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 10069-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10069-1-AP (RB1CC1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 10069-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 10069-1-AP (RB1CC1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 10069-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10069-1-AP (RB1CC1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 10069-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 10069-1-AP (RB1CC1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 10069-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 10069-1-AP (RB1CC1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 10069-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 10069-1-AP (RB1CC1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 10069-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10069-1-AP (RB1CC1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 10069-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 10069-1-AP (RB1CC1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human breast cancer using 10069-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 10069-1-AP (RB1CC1 Antibody) at dilution of 1:50.

• 

  IHC staining of human kidney using 10069-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 10069-1-AP (RB1CC1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 10069-1-AP

  Immunofluorescent analysis of HeLa cells using 10069-1-AP (RB1CC1 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: MCF7 cells, HEK-293-Zellen, HeLa-Zellen, Maus-Skelettmuskelgewebe, NIH/3T3-Zellen
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe, humanes Nierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IF: See 1 publications below

Produktinformation

10069-1-AP bindet in WB, IHC, IF/ICC, ELISA RB1CC1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RB1CC1 fusion protein Ag0003
• Vollständiger Name: RB1-inducible coiled-coil 1
• Berechnetes Molekulargewicht: 1594 aa, 183 kDa
• Beobachtetes Molekulargewicht: 183 kDa
• GenBank-Zugangsnummer: BC017556
• Gene symbol: RB1CC1
• Gene ID (NCBI): 9821
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RB1CC1, also named as RBICC, is implicated in the regulation of RB1 expression and functions as a DNA-binding transcription factor. It is a potent regulator of the RB1 pathway and a mediator that plays a crucial role in muscular differentiation. Its expression is, thus, a prerequisite for myogenic differentiation. Involved in autophagy. RB1CC1 is required for autophagosome formation. It is probably involved in the tumorigenesis of breast cancer. RB1CC1 is frequently mutated in breast cancer and shows characteristics of a classical tumor suppressor gene. This antibody is a rabbit polyclonal antibody raised against residues near the C terminus of human RB1CC1.

Publikationen

Species	Application	Title
human	WB	Proc Natl Acad Sci U S A; 26S proteasomes become stably activated upon heat shock when ubiquitination and protein degradation increase.; Authors - Donghoon Lee
human	IF	Dev Cell; The RAB11A-Positive Compartment Is a Primary Platform for Autophagosome Assembly Mediated by WIPI2 Recognition of PI3P-RAB11A.; Authors - Claudia Puri