RB1 Monoklonaler Antikörper

RB1 Monoklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

CloneNo.

1A2A6

Kat-Nr. : 67521-1-Ig

Synonyme

OSRC, p105 Rb, pp110, pRb, RB, RB1, retinoblastoma 1

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Galerie der Validierungsdaten

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  WB analysis of Jurkat using 67521-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 67521-1-Ig (RB1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 67521-1-Ig

  K-562 cells were subjected to SDS PAGE followed by western blot with 67521-1-Ig (RB1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 67521-1-Ig

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 67521-1-Ig (RB1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 67521-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 67521-1-Ig (RB1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 67521-1-Ig

  K-562 cells were subjected to SDS PAGE followed by western blot with 67521-1-Ig (RB1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HL-60 using 67521-1-Ig

  HL-60 cells were subjected to SDS PAGE followed by western blot with 67521-1-Ig (RB1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of TF-1 using 67521-1-Ig

  TF-1 cells were subjected to SDS PAGE followed by western blot with 67521-1-Ig (RB1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of Insulinoma using 67521-1-Ig

  Immunohistochemical analysis of paraffin-embedded Insulinoma tissue slide using 67521-1-Ig (RB1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of Insulinoma using 67521-1-Ig

  Immunohistochemical analysis of paraffin-embedded Insulinoma tissue slide using 67521-1-Ig (RB1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of SH-SY5Y using 67521-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using RB1 antibody (67521-1-Ig, Clone: 1A2A6 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of U2OS using 67521-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using RB1 antibody (67521-1-Ig, Clone: 1A2A6 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen, HL-60-Zellen, K-562-Zellen, MCF-7-Zellen, TF-1-Zellen
• Erfolgreiche Detektion in IHC: Insulinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: SH-SY5Y-Zellen, U2OS-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunhistochemie (IHC): IHC : 1:500-1:2000
• Immunfluoreszenz (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

67521-1-Ig bindet in WB, IHC, IF, ELISA RB1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: RB1 fusion protein Ag22578
• Vollständiger Name: retinoblastoma 1
• Berechnetes Molekulargewicht: 928 aa, 106 kDa
• Beobachtetes Molekulargewicht: 110 kDa
• GenBank-Zugangsnummer: BC039060
• Gene symbol: RB1
• Gene ID (NCBI): 5925
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RB1, also named as pp110, pRb and p105 Rb, belongs to the retinoblastoma protein (RB) family. It is a key regulator of entry into cell division that acts as a tumor suppressor. RB1 acts as a transcription repressor of E2F1 target genes. The underphosphorylated, active form of RB1 interacts with E2F1 and represses its transcription activity, leading to cell cycle arrest. It is directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation. It recruits and targets histone methyltransferases SUV39H1, SUV420H1 and SUV420H2, leading to epigenetic transcriptional repression. RB1 controls histone H4 'Lys-20' trimethylation and inhibits the intrinsic kinase activity of TAF1. It mediates transcriptional repression by SMARCA4/BRG1 by recruiting a histone deacetylase (HDAC) complex to the c-FOS promoter. In resting neurons, transcription of the c-FOS promoter is inhibited by BRG1-dependent recruitment of a phospho-RB1-HDAC1 repressor complex. Upon calcium influx, RB1 is dephosphorylated by calcineurin, which leads to release of the repressor complex. In case of viral infections, interactions with SV40 large T antigen, HPV E7 protein or adenovirus E1A protein induce the disassembly of RB1-E2F1 complex thereby disrupting RB1's activity.