RASSF7 Polyklonaler Antikörper

RASSF7 Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 21753-1-AP

Synonyme

C11orf13, HRAS1, HRC1, RASSF7

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Galerie der Validierungsdaten

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  WB analysis using 21753-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21753-1-AP (RASSF7 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 21753-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 21753-1-AP (RASSF7 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human lung cancer using 21753-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 21753-1-AP (RASSF7 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 21753-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 21753-1-AP (RASSF7 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human thyroid cancer using 21753-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 21753-1-AP (RASSF7 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human thyroid cancer using 21753-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 21753-1-AP (RASSF7 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HL-60-Zellen, HepG2-Zellen, PC-12-Zellen
• Erfolgreiche Detektion in IHC: humanes Lungenkarzinomgewebe, humanes Schilddrüsenkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

21753-1-AP bindet in WB, IHC, ELISA RASSF7 und zeigt Reaktivität mit human, Ratten

• Getestete Reaktivität: human, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RASSF7 fusion protein Ag16123
• Vollständiger Name: Ras association (RalGDS/AF-6) domain family (N-terminal) member 7
• Berechnetes Molekulargewicht: 373 aa, 40 kDa
• Beobachtetes Molekulargewicht: 40 kDa
• GenBank-Zugangsnummer: BC106921
• Gene symbol: RASSF7
• Gene ID (NCBI): 8045
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RASSF7, previously known as HRC1 (HRAS1 cluster 1) and C11orf13, is a member of the N-terminal RASSF family. RASSF7 protein localises to the centrosome and plays a key role in mitosis. RASSF7 is expressed in a broad range of different cell types and that this expression could be enhanced following exposure to hypoxia. RASSF7 has three isoforms with the MW 40 kDa, 36 kDa and 35 kDa.